重组人骨形成蛋白-2-珊瑚复合人工骨修复骨缺损的生物力学研究

A Biomechanical Study on the Bone Repairing Ability of Recombinant Human Bone Morphogenetic Protein 2 Coral Composited Artificial Bone

将珊瑚与具有骨诱导特性的重组人骨形成蛋白－２（ｒｈＢＭＰ－２）复合，制成ｒｈＢＭＰ－２－ｃｏｒａｌ复合人工骨，将其植入兔颅骨标准大小缺损，并与单纯珊瑚植入作对照。通过Ｘ线片、组织学和生物力学方法来评价此复合人工骨的骨修复能力。结果显示：ｒｈＢＭＰ－２－ｃｏｒａｌ复合人工骨具有较强的骨修复作用，植入骨缺损后，材料被逐渐降解吸收，新骨不断形成，机械强度不断增大；１２周后，植入物完全被成熟的骨组织取代，缺损区抗压强度接近正常的骨组织。该复合人工骨是一种较理想的骨移植替代材料。

To improve bone repairing ability of coral, recombinant human bone morphogenetic protein 2 (rhBMP 2) coral composited artificial bone was obtained by combining coral with rhBMP 2 in this experiment. The composite was implanted into rabbit calvarial critical size defects and coral alone was implanted as control. The bone repairing ability was assessed by radiography, histology and biomechanics. The results showed that implants were absorbed gradually after they was implanted into the defects. In the meantime, the new bone was formed and the mechanical strength was reinforced uninterruptedly in bone defect regions. At 12 weeks, the implants were replaced completely by bone, and the mechanical strength was entirely resored. These suggest that the composite possess a superior bone repairing ability and may be one of the most perfect bone graft substitutes currently available.