摘要
目的：研究Ｕ７３１２２对细胞内钙离子浓度和电压依赖性钙通道的作用．方法：用Ｆｕｒａ２荧光测定胞浆钙浓度和用穿孔膜片箝记录全细胞钙电流．结果：Ｕ７３１２２呈剂量相关明显地降低ＲＩＮｍ５Ｆ细胞与子宫平滑肌细胞的去极化诱导的钙电流，并抑制ＫＣｌ诱导的与ＢａｙＫ８６４４诱导的胞浆钙浓度的增加．Ｕ７３１２２的这种作用对子宫平滑肌细胞要比ＲＩＮｍ５Ｆ细胞强，而一种非磷脂酶Ｃ抑制剂Ｕ７３１２２类似物Ｕ７３３４３也能降低钙电流５０％．结论：Ｕ７３１２２抑制ＲＩＮｍ５Ｆ细胞与猪子宫平滑肌细胞的电压依赖性钙通道和胞浆钙浓度．
AIM: To study the effect of U 73122, putative phospholipase C (PLC) inhibitor, on cytosolic Ca 2+ concentration ([Ca 2+ ] i) and voltage dependent calcium channels (VDCC). METHODS: To record whole cell calcium current ( I Ca ) by perforated patch clamp and to measure cytosolic free calcium concentration ([Ca 2+ ] i) with Fura 2. RESULTS: U 73122 inhibited KCl induced and Bay K 8644 induced increase in [Ca 2+ ] i dose dependently. U 73122 decreased markedly depolarization induced I Ca both in RINm5F and porcine myometrial cells in a concentration dependent manner. The effect of U 73122 was more potent in porcine myometrial cells than that in RINm5F cells. U 73343 (an analog of U 73122 ), that does not inhibit PLC, also reduced the I Ca by 50 % . CONCLUSION: U 73122 inhibited VDCC and [Ca 2+ ] i in both RINm5F and porcine myometrial cells.
出处
《中国药理学报》
CSCD
1997年第5期385-390,共6页
Acta Pharmacologica Sinica
关键词
Bay-K-8644
钙通道
胰岛
子宫平滑肌
U-73122
Bay K 8644
calcium
calcium channels
Fura 2
islets of Langerhans
myometrium
patch clamp techniques
phospholipase C
U 73122
U 73343
