摘要
目的:检测前阿黑皮原(POMC)和强啡肽原(PPD)基因在高血压(SHR)和同龄WistarKyoto大鼠(WKY)中的表达.方法:用地高辛(DIG)标记的RNA探针进行原位杂交检测POMCmRNA和PPDmRNA.结果:POMCmRNA主要表达于弓状核,而SHR表达量大于WKY.PPDmRNA表达于海马、下丘脑、中脑中央灰质、孤束核、胸髓.在齿状回、孤束核、内侧视前区,SHRPPDmRNA表达量少于WKY;在弓状核、胸髓、海马CA1、CA2、CA3两者之间无差别.结论:SHR弓状核POMCmRNA增加和齿状核等PPDmRNA减少可能与高血压的发病有关.
AIM: To compare the expressions of prepropiomelanocortin (POMC) mRNA and preprodynorphin (PPD) mRNA between 16 wk old spontaneously hypertensive rats (SHR) and age matched normotensive Wistar Kyoto rats (WKY). METHODS: The expression of POMC mRNA and PPD mRNA were detected with nonradioactive in situ hybridization by digoxigenin labeled RNA probe. RESULTS: POMC mRNA mainly was expressed in arcuate nucleus, compared with WKY, SHR had higher level of POMC mRNA (542). PPD mRNA was found in hippocampus, hypothalamus, central gray, nucleus of the solitary tract (NTS), and thoracic spinal cord ( T4-T6 ). Compared with WKY, PPD mRNA level of SHR decreased in dentate gyrus (2342), NTS (381), and medial preoptic area (467); no difference was observed in arcuate nucleus (263), thoracic spinal cord (750-1800) and CA1, CA2, CA3 of hippocampus (1674, 2014, 2626). CONCLUSION: Increase of POMC mRNA in arcuate nucleus and decrease of PPD mRNA in dentate gyrus of SHR may be associated with the genesis of spontaneous hypertension.
出处
《中国药理学报》
CSCD
1997年第5期391-394,共4页
Acta Pharmacologica Sinica
关键词
高血压
前阿黑皮原
强啡肽
原位杂交
脑
hypertension
preproopiomelanocortin
dynorphins
messenger RNA
in situ hybridization
brain
inbred SHR rats
inbred WKY rats