生长抑素和电针抑制关节炎大鼠脊髓c-fos的表达

Somatostatin and electroacupuncture inhibited c fos expression in spinal cord of arthritic rats

目的：观察鞘内注射生长抑素及电针“夹脊”穴对病理性疼痛情况下大鼠脊髓ｃｆｏｓ原癌基因蛋白表达的影响．方法：用急性佐剂性关节炎大鼠作为病理性疼痛的实验模型，免疫组织化学法检测ｃｆｏｓ样免疫反应细胞．结果：佐剂性关节炎可诱发大鼠脊髓内多层神经元ｃｆｏｓ蛋白表达，接种后２４ｈ，以背角Ⅰ－Ⅱ，Ⅴ－Ⅵ层内最多，而生长抑素和电针均可抑制其表达，表现为ｃｆｏｓ蛋白形成减少．结论：生长抑素和电针缓解病理性疼痛的作用与抑制伤害性痛反应诱发的脊髓ｃｆｏｓ表达有关．

AIM: To observe the effect of intrathecal injection of somatostatin (Som) associated with electroacupuncture (EA) at “Jiaji” points on c fos protein expression of spinal cord in pain rats. METHODS: Rats with adjuvant arthritis were used as pain model and the c fos like immunoreactivity (FLI) was detected by immunohistochemistry. RESULTS: The c fos protein expression induced by arthritis were found in all of the Ⅰ-Ⅹ laminae of the ipsilateral spinal cord of rats, and most of the labeled cells were located in the laminae Ⅰ-Ⅱ and Ⅴ-Ⅵ. Som and EA suppressed the c fos expression and the lessening of FLI cells in the spinal cord. CONCLUSION: Pathological pain following arthritis activated pain sensitive neurons (PSN) and evoked c fos expression in spinal cord, Som and EA supressed activities of these PSN, producing the effect of analgesia.