穿心莲成分API_(0134)对动脉粥样硬化家兔血清一氧化氮、血浆内皮素和脂质过氧化物的影响

Effects of Andrographis Paniculata Component on Nitric Oxide, Endothelin and Lipid Peroxidation in Experimental Atheroscierotic Rabbits

目的：观察穿心莲成分ＡＰＩ０１３４预防用药对动脉粥样硬化（ＡＳ）家兔血清一氧化氮（ＮＯ），血浆内皮素（ＥＴ）、环鸟苷酸（ｃＧＭＰ）、脂质过氧化物（ＬＰＯ）和超氧化物歧化酶（ＳＯＤ）的影响。方法：采用高胆固醇饲料喂饲另加牛血清白蛋白一次注射方法，建立家兔ＡＳ模型，随机分为对照组，模型组和ＡＰＩ０１３４用药组，各组在实验第４周和第８周取血标本测定上述参数。结果：与模型组比较，穿心莲成分ＡＰＩ０１３４预防用药４周及８周，均能提高ＮＯ、ｃＧＭＰ含量和ＳＯＤ活性（Ｐ＜０．０１），降低ＥＴ和ＬＰＯ含量（Ｐ＜０．０１）。结论：ＡＰＩ０１３４具有抗氧化、保护内皮功能和维持ＮＯ／ＥＴ平衡的作用。

To observe the effects of Andrographis Paniculata component(API0134) on nitric oxide(NO), endothelin (ET), cyclic guanosine monophosphate(cGMP), lipid peroxide (LPO) and superoxide dismutase (SOD) inexperimental atherosclerotic rabbit. Mathods: An atherosclerotic rabbit model was established by feeding highcholesterol diet supplemented by bovine serum albumin injection bolus. The rabbits were randomly divided into thecontrol, model, and API0134 treated group. Blood samples were collected before 4 weeks and 8 weeks after releventtreatment. Results: Before 4 and after 8 weeks API0134 administration, compared with model group, the NO, cGMPand activity of SOD increased (P<0.01), while LPO and ET decreased(P<0.01). Conclusions: API0134 possessesthe effects of antioxidation, preseting endothelial function,and maintaining the balance of NO/ET.