PD4、CD44v6及CD44v9蛋白在人喉癌组织中的表达及临床意义

Expression of PD4,CD44v6,CD44v9 and its clinical significance in human laryngeal carcinoma

目的：探讨支原体单克隆抗体PD4与黏附分子CD44v6、CD44v9蛋白在人喉癌组织中的表达及临床意义。方法：采用免疫组织化学Envision法检测212例喉不同病变组织标本（喉癌137例,喉癌前病变26例,声带息肉34例和癌周正常喉组织15例）中PD4、CD44v6、CD44v9蛋白的表达。结果：①PD4、CD44v6、CD44v9在人喉癌组织中表达阳性率分别为45.3%（62/137）、72.3%（99/137）、56.2%（77/137）,明显高于其他喉不同病变组织（均P〈0.01）。②中晚期（Ⅲ～Ⅳ期）、伴颈淋巴结转移的喉癌组织中PD4、CD44v6、CD44v9阳性表达率均显著高于早期（Ⅰ～Ⅱ期）和无颈淋巴结转移的喉癌组织（均P〈0.01）;喉癌术后3年或5年存活患者PD4、CD44v6、CD44v9表达阳性率明显低于生存时间未达3～5年者（P〈0.01或P〈0.05）。③PD4和CD44v6、CD44v9在人喉癌组织中联合表达阳性率为27.7%（38/137）,显著高于喉其他不同病变组织中联合表达阳性率（5.3%,4/75）（P〈0.01）。结论：喉癌的发生、发展及预后与PD4、CD44v6、CD44v9联合表达密切相关。

Objective：Study on the role of mycoplasma infection and expression of CD44v6,CD44v9 protein in the pathogenesis,development and prognosis of laryngeal carcinoma（LC）.Method：Immunohistochemistry was used to study 137 cases of laryngeal carcinoma tissues,26 cases of precarcinoma tissues,34 cases of vocal cord polypus and 15 cases of normal tissues adjacent to laryngeal carcinoma.Result：①The positive rate of PD4 and CD44v6,CD44v9 was 45.3%（62/137）,72.3%（99/137） and 56.2%（77/137） in laryngeal carcinoma,which are much higher than those in non-carcinoma tissues.②The positive rate of PD4 and CD44v6、CD44v9 in the third and fourth stages of LC were higher than those in the first and second stages of LC.Laryngeal carcinoma with cervical metastasis had higher expression than those without cervical metastasis（P〈0.01）.To 3 and 5 years survival,PD4 and CD44v6,CD44v9 positive cases had lower chance than those negative cases（P〈0.01或0.05）.③The over all positive rate of PD4 and CD44v6,CD44v9 was 27.7%（38/137） in laryngeal carcinoma tissues and 5.3%（4/75） in non-carcinoma tissues.Conclusion：These results implicates that the pathogenesis,development and prognosis of laryngeal carcinoma maybe closely related to the high expression of PD4 and CD44v6,CD44v9 protein.