胸苷磷酸化酶、中期因子及CD105在喉癌组织中的表达及临床意义

The expressions of TP,MK and CD105 in laryngeal squamous cell carcinoma and their clinical significance

目的:探讨胸苷磷酸化酶(TP)、中期因子(MK)和CD105在喉鳞状细胞癌组织中的表达及临床意义。方法:采用免疫组织化学方法检测43例喉鳞状细胞癌组织中TP、MK的表达和CD105标记的微血管密度(MVD)情况。结果:TP的阳性表达率为67.4%,MK的阳性表达率为60.5%,CD105标记的MVD值为6.01±1.78。TP和MK均为阳性时CD105标记的MVD值(7.07±3.26)明显高于TP和MK均为阴性时的MVD值(4.03±1.90)(P<0.05)。三者表达均与临床分期、淋巴结转移有关。单因素分析显示TP、MK表达和高MVD(≥6)均影响喉癌患者的生存期,但通过多因素分析显示只有淋巴结转移和MVD是影响喉癌患者生存期的预后因素。结论:TP、MK表达与喉癌血管生成关系密切,是喉癌肿瘤血管生成的重要因子,三者与喉癌进展、淋巴结转移密切相关。淋巴结转移和CD105标记的MVD是影响喉癌预后的因素;TP、MK可能通过促肿瘤血管生成作用对喉癌预后产生影响;TP、MK及CD105可作为一组有价值的肿瘤标记和预后指标。

Objective：To explore the expressions of thymidine phosphorylase（TP）,midkine（MK） and MVD marked with CD105 antibody in laryngeal squamous cell carcinoma（LSCC） and their clinical significance.Method：The expressions of TP,MK and CD105 in LSCC tissues of 43 cases were studied by immunohistochemical staining.Result：The positive expression rates of TP and MK in LSCC were 67.4% and 60.5% respectively,the mean vaule of MVD was 6.01±1.78.MVD was significantly higher in tumor tissue with both positive TP and MK than in that with both negative TP and MK（7.07±3.26 vs 4.03±1.90,P〈0.05）.The expression of TP,MK and CD105 were all correlated with T-stage and lymph node metastasis.Positive TP,MK expression and high MVD were all associated with a poor survival,and positive expression of both TP and MK in tumors conferred a poorer prognosis than negative expression of those factors in tumors,but only the lymph node metastasis and MVD were independent prognostic factors on multivariate analysis.Conclusion：Both TP and MK are important for angiogenesis in LSCC.TP,MK and angiogenesis are all closely correlated with the progress of LSCC and the lymph node metastasis.The lymph node metastasis and MVD marked with CD105 antibody were independent prognostic factors.TP and MK may affect the progression and prognosis of tumor by promotion of angiogenesis.A combinative detection of TP,MK and CD105 can be as valuable tumor marker and prognostic factor for LSCC.