基质金属蛋白酶11在喉癌中的表达

Expression and clinical significance of stromelysin-3 in laryngeal cancer

目的:检测喉癌中基质金属蛋白酶11(MMP11)的表达,探讨MMP11的表达与喉癌浸润和转移的关系。方法:采用免疫组织化学法检测63例喉癌组织和相应的癌旁正常组织MMP11蛋白的表达,运用RT-PCR技术检测22例喉癌和相应的癌旁正常组织冻存标本中MMP11 mRNA水平的表达。结果:采用免疫组织化学法检测喉癌和癌旁正常组织MMP11的表达,癌组织MMP11的表达阳性率明显高于癌旁正常组织(P<0.01)。运用RT-PCR方法检测喉癌和癌旁正常组织MMP11 mRNA的表达,癌组织MMP11 mRNA的表达阳性率明显高于癌旁正常组织(P<0.01)。MMP11蛋白表达在T3+T4组高于T1+T2组(P<0.01),淋巴结转移组高于非淋巴结转移组(P<0.05)。MMP11 mRNA表达在T3+T4组高于T1+T2组(P<0.05),淋巴结转移组高于非淋巴结转移组(P<0.05)。MMP11蛋白及MMP11 mRNA的表达与肿瘤原发部位和分化程度无明显相关性(P>0.05)。结论:MMP11的表达与喉癌浸润和转移密切相关,可作为预测喉癌浸润和转移潜能的参考指标之一。

Objective：To investigate the role of expression of stromelysin-3 in laryngeal carcinoma and the relationship between stromelysin-3 expression and the invasion and metastasis of laryngeal cancer.Method：The expression of stromelysin-3 in 63 samples with laryngeal carcinoma and 63 adjacent normal tissue were detected by immunohistochemistry.The mRNA expression of stromelysin-3 were examined by reverse transcript-polymerase chain reaction（RT-PCR） in 22 frozen specimens of laryngeal cancer and 22 adjacent normal tissue.Result：The immunochemistry showed the expression of stromelysin-3 in laryngeal cancer is much higher than that in adjacent normal tissue（P〈0.01）,RT-PCR showed the expression of stromelysin-3 in laryngeal cancer was much higher than that in adjacent normal tissue（P〈0.01）.The expression of stromelysin-3 in protein level was not only much higher in T3＋T4 group than that in T1＋T2 group（P〈0.01） but also much higher in nodal metastases group than that in the group without nodal metastases（P〈0.05）.The mRNA expression of stromelysin-3 was higher in T3＋T4 group than that in T1＋T2 group（P〈0.05） and was higher in nodal metastases group than that in group without nodal metastases（P〈0.05）.The expression of stromelysin-3 both in protein level and in mRNA level was not associated with different primary location and different histological grade of laryngeal cancer（P〉0.05）.Conclusion：The expression of stromelysin-3 was closely associated with the invasion and metastases of laryngeal carcinoma,it may be served as a marker in estimating the invasion and metastases potency of laryngeal cancer.