喉癌手术切缘中Survivin和Caspase-3及p53的表达意义及相关性研究

Expression of Survivin,Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma and their relationship

目的：研究Survivin、Caspase-3及p53在喉鳞状细胞癌手术切缘中的表达意义及相关性。方法：116例患者术后按肿瘤复发情况分成4组,分别为：复发≤1年组,〉1～3年组,〉3～〈5年组,≥5年组（包括5年后无复发）,应用免疫组织化学方法检测手术切缘Survivin、Caspase-3及p53的表达情况。结果：Survivin在4组中的表达分别为92.9%、85.0%、50.0%及21.1%（P〈0.01）;Caspase-3在4组中的表达分别为50.0%、50.0%、66.7%及89.5%（P〈0.01）;p53在4组中的表达分别为57.1%、37.5%、45.8%及18.4%（P〈0.05）;Survivin、Caspase-3及p53在4组的表达均有统计学意义。结论：喉癌手术切缘中Survivin、Caspase-3及p53的表达在喉鳞状细胞癌的发生、发展中可能起重要作用,并对喉鳞状细胞癌的早期诊断和预后判断有意义。

Objective：To study the expression of Survivin, Caspase-3 and p53 in surgical margin of laryngeal squamous cell carcinoma（LSCC）,and their clinical significiance and relationship.Method：Immunohistochemistry method was used to detect the expression of Survivin,Caspase-3 and p53 in 116 cases of surgical margin of LSCC.Result：According to the recurrence,the patients were divided into four group,recurrence within one year,between one year to three years,between three to five years,more than 5 years（include no recurrence）.The expression rate of survivin were 92.9%,85.0%,50.0% and 21.1%（P〈0.01） in four groups,respectively.The expression rate of Caspase-3 were 50.0%,50.0%,66.7% and 89.5%（P〈0.01）.The expression rate of p53 were 57.1%,37.5%,45.8% and 18.4%（P〈0.05）.The expression rate of survivin,caspase-3 and p53 in four groups,respectively,there were significant differences（P〈0.05）.Conclusion：Expression of Survivin,Caspase-3 and p53 in surgical margin of LSCC may play an important role in the tumorigenesis and develeopment of laryngeal carcinoma.They are feasible for early diagnosis and prognosis estimation.