槲皮素对人喉鳞状细胞癌细胞系Hep-2的作用

Effects of Quercetin on human laryngeal cancer Hep-2 cells

目的:探讨槲皮素对体外培养的人喉鳞状细胞癌细胞系Hep-2生长的影响及作用机制,研究槲皮素治疗喉癌的可行性。方法:采用MTT法?倒置相差显微镜及流式细胞仪观察槲皮素对人喉鳞状细胞癌细胞系Hep-2的作用情况。结果:槲皮素呈时间和浓度依赖性抑制Hep-2细胞的增殖,使Hep-2细胞贴壁能力减弱,形态变小变圆,悬浮细胞和颗粒增加,引起Hep-2凋亡,细胞周期阻滞于S期。结论:槲皮素能够抑制喉鳞状细胞癌细胞系Hep-2的生长和增殖,其抑制作用与细胞凋亡有关。

Objective： To investigate the antiproliferative effects of quercetinon on human laryngeal cancer Hep-2 cells and its mechanism. Method： The antiproliferative effects of quereetinon on human laryngeal cancer Hep-2 cells was evaluated by MTT assay; The morphological alterations were studied by inverted phase contrast microscope. The apoptosis and cell cycle of Hep-2 cells was analyzed by flow cytometry （FCW）, Result： The growth of Hep-2 cells was significantly inhibited by quercetin. By inverted phase contrast microscope, morphological alterations including adherencing capability weakening and shape shrinking were noticed. It was found that apoptosis cells increased with the increase of drug concentration and.the elapse of time by FCW. Cell cycle transition arrested at S phase and the broken DNA was noticed. Conclusion： Quercetin could effectively inhibit the proliferation of Hep-2 cells and its mechanism is probably related to the apoptosis.