三氧化二砷和维生素C联合诱导喉癌细胞Hep-2凋亡的作用

Effects of vitamin C comined with arsenic trioxide on the apoptosis of Hep-2 cell

目的:研究三氧化二砷(As2O3)与维生素C联用对喉癌细胞(Hep-2)凋亡的协同作用。方法:As2O3及联用VitC孵育体外培养的人Hep-2,采用四甲基偶氮唑蓝(MTT)法测定细胞存活率,Annexin-V/PI双染色流式细胞术观察各组细胞凋亡变化。结果:As2O3与VitC联用能显著降低单用As2O3的细胞存活率(P<0.05),同时VitC能显著增强As2O3诱导细胞凋亡作用(P<0.01)。结论:联用VitC能增强As2O3诱导Hep-2凋亡的作用。

Objective： To study synergistic effect of Vitamin C （VitC） combined with arsentic trioxide （As2O3）-on human laryngocarcinoma Hep-2 cell line apoptosis. Method： Human laryngocarcinoma cell line Hep-2 was cultured in vitro, and incubated with As2O3 or jointly with VitC. The inhibition ability of Hep-2 cells was detemined by methyl thiazolyl tetrazolium （MTT） assay, while the apoptosis was mensurated by the flow cytometry with Annexin-V/PI. Result：Compared with the use of As2O3 separately, the combination of As2O3 and VitC could increase the inhibition ability and the apoptosis rate of Hep-2 cells markedly （P〈0.05）. Conclusion： Combination of As2 O3 with VitC could markedly increase arsentic trioxide （As2 O3）-induced apoptosis of Hep-2 cells.