单核细胞趋化蛋白-3在喉鳞状细胞癌中的表达及临床意义

Expression and distribution of monocyte chemotactic protein-3 and its significance in laryngeal carcinoma

目的:明确单核细胞趋化蛋白-3(MCP-3)在喉鳞状细胞癌及癌旁正常组织的表达及分布,并探讨其在喉癌发病机制中的作用及临床意义。方法:采用RT-PCR,Westernblot及免疫组织化学技术检测MCP-3在喉癌组织和癌旁正常组织中的表达和分布。结果:RT-PCR检测表明,MCP-3mRNA在喉癌及喉正常组织内均有表达,MCP-3在喉癌组织中的表达较癌旁正常组织表达明显增多(P<0.05),并且在喉癌组织Ⅰ～Ⅱ期与Ⅲ～Ⅳ期表达差异有统计学意义(P<0.05);蛋白免疫印迹检测发现,喉癌组织和癌旁正常组织内均有MCP-3表达,MCP-3的表达位置在相对分子质量11000;喉癌组织中MCP-3的表达明显较癌旁正常组织的表达增多(P<0.01),并且在喉癌组织Ⅰ～Ⅱ期与Ⅲ～Ⅳ期表达之间差异有统计学意义(P<0.05);免疫组织化学染色发现:MCP-3阳性表达为黄色,主要分布在肿瘤细胞细胞质内,在喉正常的腺上皮组织中也可检测到阳性表达。结论:MCP-3可能与喉癌的发病机制有关,可能与肿瘤相关巨噬细胞的趋化和激活有关,肿瘤内的炎性微环境对肿瘤的发生、发展起重要作用。

Objective：To investigate monocyte chemotactic protein-3 （MCP-3） mRNA and protein expression and distribution in laryngeal squamous cell carcinoma （LSCC） tissue, so as to study its function in the tumor development. Metbod：Immunohistochemical staining and RT PCR, and western blot were performed to detect the LSCC tissue and the normal tissue adjacent tumor. Result：The expression of MCP-3 mRNA and protein were found in the LSCC tissue and the normal tissue adjacent tumor. The expression of MCP-3 mRNA and protein in LSCC tissue is much higher than that in normal tissue adjacent tumor （ P〈0. 05）. There is a significant difference between Ⅰ～Ⅱ stage Ⅲ～Ⅳ stage （ P〈0.05）. Immunohistochemical staining： positive staining of MCP-3 was yellow and mainly detected in the tumor cell cytoplasm and also in some normal glandular epitheliums. Conclusion： MCP-3 might be involved in the mechanism of the tumor development, its function of chemotaxis and activation of the tumor-associated macrophages would be highlighted, so that the inflammatory microenvironment might play an important role in the development of tumor.