我国沿海三疣梭子蟹9个野生群体线粒体CR和COⅠ片段比较分析

Compared Analysis on the Sequences of Mitochondrial CR Gene and COⅠ Gene Fragments of Nine Wild Stocks of Portunus trituberculatus from the Marginal Seas of China

通过对我国沿海三疣梭子蟹（Portunus trituberculatus）9个野生群体线粒体DNA控制区（putative control region,CR）基因片段和细胞色素氧化酶亚基Ⅰ（COⅠ）基因片段进行扩增和测序,分别得到长度为530bp和584bp的片段。分析结果表明：CR83条序列A＋T平均含量为73.2%,共检测到91个变异位点,83个个体具有66种单倍型（haplotype） COⅠ94条序列A＋T平均含量为62.2%,共检测到34个变异位点,94个个体具有34种单倍型（haplotype）。用MEGA3.1软件构建9个群体NJ分子树,聚类结果显示,黄海、东海、渤海6个群体之间的相对遗传距离比较近,聚为一大支,南海3个群体相对遗传距离比较近,聚为一大支。AMOVA分析表明,群体间的遗传分化指数（Fst）为-0.07454-0.27087,其中部分群体间达到显著差异（P〈0.05）与极显著差异（P〈0.01）,说明我国三疣梭子蟹不同野生群体间存在一定遗传分化。

Mitochondrial CR gene and CO Ⅰ gene fragments were amplified and sequenced from 9 wild stocks of Portunus trltuberculatus in China. Respectively ,530 bp and 584 bp long partial gene fragments of the CR gene and the CO Ⅰgene were obtained. A ＋ T mean contents of the CR sequences and the CO Ⅰ sequences were respectively 73.2% and 62.2%. Moreover,66 haplotypes were identified from 83 samples with 91 variation sites of CR gene and 33 haplotypes were identified from 96 samples with 42 variation sites of COⅠ gene. The genetic distance and cluster analysis showed that genetic distances were nearest among six stocks of Yellow Sea, East China Sea, Bohai Sea, while the genetic distances were nearest among three stocks of South China Sea. The fixation indices （ Fst ） were from - 0.074 54 to 0.270 87 between any two stocks and some of them got to significant and great significant level. So these indicated that there were some genetic differentiations in different wild stocks of P. trituberculatus in China.