摘要
采用５′非编码区的引物，用逆转录聚合酶链反应（ＲＴＰＣＲ）检测１００名献浆血员中庚型肝炎病毒ＨＧＶＲＮＡ，其中１９名（１９％）ＨＧＶＲＮＡ阳性；对其中４份ＰＣＲ产物进行了直接测序，结果表明４株病毒在此区的核苷酸序列相对保守，其同源性在９５％以上，与美国报道的ＨＧＶ和ＧＢＶＣ的同源性分别为８８％和８５％不同。提示该４株ＨＧＶ与美国报道的ＨＧＶ和ＧＢＶＣ不属于同一基因型。
Hepatitis G virus(HGV) was detected in plasma samples from blood donors by RT PCR with 5′ noncoding region′s primers of HGV.The results showed that 5 of 20 from Beijing,8 of 40 from Heilongjiang province,2 of 20 from Sichuan province and 4 of 20 from Jilin province were positive for HGV RNA,with the prevalence rate of 19%(19/100).In addition,4 of 19 HGV RNA positive PCR products were sequenced directly.The identity of the nucleic acid between four Chinese isolates was more than 95% and 88% or 85% between Chinese isolates and HGV or GBV C reported in the United States.The data showed that these four Chinese isolates may belong to a new genotype of HGV,different form the GBV C and HGV reported in the United States.
