摘要
采用5′非编码区的引物,用逆转录聚合酶链反应(RTPCR)检测100名献浆血员中庚型肝炎病毒HGVRNA,其中19名(19%)HGVRNA阳性;对其中4份PCR产物进行了直接测序,结果表明4株病毒在此区的核苷酸序列相对保守,其同源性在95%以上,与美国报道的HGV和GBVC的同源性分别为88%和85%不同。提示该4株HGV与美国报道的HGV和GBVC不属于同一基因型。
Hepatitis G virus(HGV) was detected in plasma samples from blood donors by RT PCR with 5′ noncoding region′s primers of HGV.The results showed that 5 of 20 from Beijing,8 of 40 from Heilongjiang province,2 of 20 from Sichuan province and 4 of 20 from Jilin province were positive for HGV RNA,with the prevalence rate of 19%(19/100).In addition,4 of 19 HGV RNA positive PCR products were sequenced directly.The identity of the nucleic acid between four Chinese isolates was more than 95% and 88% or 85% between Chinese isolates and HGV or GBV C reported in the United States.The data showed that these four Chinese isolates may belong to a new genotype of HGV,different form the GBV C and HGV reported in the United States.