摘要
目的研究血管抑制因子METH1基因转染对兔耳瘢痕成纤维细胞增殖、胶原合成的影响。方法复制兔耳增生性瘢痕模型，用微循环显微镜检、瘢痕组织AgNOR染色、苦味酸-天狼星红染色等方法，观察基因重组血管抑制剂Ad-METH1对增生性瘢痕组织血管生成、成纤维细胞增殖、胶原分布的影响。结果Ad-METH1注射后30d瘢痕组织血管生成明显减少，成纤维细胞增殖减弱、Ⅰ／Ⅲ型胶原比明显降低。结论上皮化后早期血管抑制基因治疗可有效抑制增生性瘢痕的形成。
Objective To investigate the effect of METH1 gene transfection on fibroblast proliferation and Ⅰ ,Ⅲcollagen synthesis in rabbit ear scar. Methods The hypertrophic scar model on the rabbit ears was reproduced. 10 days after epithelization, Ad-METH1 was injected into the scar tissue. 30 days later, the effect of METH1 gene transfection on the angiogenesis, fibroblast proliferation and the ratio of collagen Ⅰ/Ⅲ in the scar tissue was detected by microcirculation microscope, AgNOR particle connt and collagen dyeing. Results 30 days after injection of Ad-METH1, angiogenesis, fibroblast proliferation and the ratio of collagen Ⅰ/Ⅲ in the scar tissue were obviously suppressed. Conclusion Early application of Ad-METH1 after epithelization can markedly inhibit the formation of the hypertrophic scar.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2008年第2期148-150,共3页
Chinese Journal of Plastic Surgery
基金
国家自然科学基金资助项目（30271346）
关键词
增生性瘢痕
新生血管化
生理性
基因治疗
Hypertophic scar
Neovascularization, physiologic
Gene therapy
