视黄酸类物质诱导HL-60细胞分化过程中CDKs激酶活性变化

Changes in Activities of Cyclin dependent Kinases in HL 60 Cells During Granulocytic Differentiation Induced by Retinoids

应用组蛋白Ｈ１激酶活性分析技术，检测视黄酶和芳维甲诱导ＨＬ６０细胞分化过程中细胞周期素依赖的蛋白激酶ＣＤＫｓ活性变化。结果显示处理３～４天的细胞大部分已向粒系分化，ＣＤＫ２和ＣＤＫ４激酶活性显著降低。推测细胞周期素依赖的蛋白激酶活性下降可能与视黄酸类物质诱导ＨＬ６０细胞分化有关。

The role of cyclin dependent kinases in differentiation of HL 60 cells has been studied by analysing the changes of cyclin E and cyclin D 1 associated kinase activities by histone H 1 kinase assays.During differentiation induction with retinoic acid and arotinoid ethylester,both cyclin E and cyclin D 1 associated kinase activities were decreased,which may partially owing to upregulation of CDK inhibitors(CKIs) that could bind and inactivate cyclin/CKD complexes.Our data suggest that inhibition of CDK activity may be relative to granulocytic differentiation of HL 60 cells.