大鼠局部脑缺血再灌流的实验研究

The experimental study on focal cerebral ischemia and reperfusion in rats

采用大鼠局部脑缺血再灌流模型，研究了大鼠脑缺血６ｈ、９ｈ和缺血６ｈ再灌流３ｈ脑梗塞体积，脑含水量，能量代谢，丙二醛（ＭＤＡ）和超氧化物歧化酶（ＳＯＤ）的变化。结果：脑缺血６ｈ、９ｈ可以造成严重的脑梗塞和脑水肿，ＡＴＰ含量和ＳＯＤ活性显著降低，乳酸和ＭＤＡ含量显著增加，和对照组相比均有显著差异（Ｐ＜０．０５或Ｐ＜０．００１）。再灌组和缺血两组比较，脑梗塞体积，脑水肿无明显差别（Ｐ＞０．０５），ＡＴＰ、乳酸、ＳＯＤ和ＭＤＡ均有不程度的改善。提示，大鼠局部脑缺血超过６ｈ可造成严重的脑损伤，并随缺血时间的再延长，脑损伤变化趋于平缓。再灌后，脑损伤未见明显加重。

Using the rat model of reversible focal cerebral ischemia,we studied the changes of cerebral infarction size,brain water content,energy metabolism,MDA and SOD in rats subjected to 6 or 9 hr of permanent cerebral ischemia and 6 hr of cerebral ischemia followed by 3 hr of reperfusion.The results showed that the severe cerebral infarction size and brain edema were caused,the content of ATP and the activity of SOD significantly decreased, the content of lactate and MDA significantly increased in the two permanent ischemia groups compared with the control group(P<0.05 or P<0.001). The changes of cerebral infarction size and brain edema were not significantly different(P>0.05) between the reperfusion group and the permanent ischemia groups. Our present data indicated that 6 hr of cerebral ischemia could induce severe brain damage,after then,the evolution of brain injury tended to be slow with increasing ischemic time.The reperfusion injury was not significant in this study.