大孔吸附树脂纯化贯叶金丝桃总黄酮的在线检测研究

Purification of total flavonoid glycosides from Hypericum perforatum by macroporous adsorption resin with on-line detection system

目的:以贯叶金丝桃总黄酮的制备为例,设计一种具有在线检测功能的大孔吸附树脂柱色谱系统。方法:经过对常规检测方法的比较分析,选择具有流动检测功能的紫外检测器为在线检测器,使用自制分流器进行连接,设计了大孔树脂分离纯化中药成分的在线检测系统,并用于贯叶金丝桃总黄酮的纯化。结果:在线检测系统操作简单,检测结果准确客观,制备得到的贯叶金丝桃总黄酮含量可达到90%以上。结论:该技术为中药提取物色谱纯化的工业化生产提供了简单易行、稳定可靠的在线检测方法,为中药纯化自动化生产奠定基础。

Objective： To design a macroporous resin chromatography system with on-line detector which can be used to purify total flavonoid glycosides from Hypericum perforatum. Method ： Compared with the present detection methods, the ultraviolet detector which can be applied to flow liquid, and a self-made shunt were applied to design the on-line detection system. This system was successfully applied to purify the total flavonoid glycosides from H. perforatum. Result： This on-line detection system was simple and feasible, and the results were accurate and reliable. The purity of total flavonoid glycosides from H. perforatum, separated by this system was higher than 90%. Conclusion： This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts, which is the basis of automatic manufactory of TCM purification.