左归丸对免疫性卵巢早衰小鼠卵巢Fas、Fas-L表达的影响

Effects of Zuogui pill on Expression of Fas/FasL Protein in Ovaries of Immune Premature Ovarian Failure Mice.

目的:探讨免疫性卵巢早衰小鼠(POF)卵巢Fas、Fas-L蛋白的表达及补肾复方左归丸的影响。方法:以小鼠透明带3为抗原,皮下多点注射免疫BALB/C雌性小鼠建立免疫性卵巢早衰模型。设左归丸低、中、高不同剂量进行治疗,以泼尼松、己烯雌酚为阳性对照,免疫组化法检测卵巢卵泡、间质Fas、FasL蛋白的表达。结果:POF小鼠大、小卵泡Fas蛋白表达均明显减少,小卵泡、间质FasL表达均明显增强。左归丸各剂量组大、小卵泡Fas表达明显增强;左归丸低剂量组间质Fas表达也增强,小卵泡FasL表达减弱;左归丸高剂量组间质Fas表达也均增强,与模型组相比差异显著。结论:POF小鼠Fas/FasL系统平衡失调,B细胞凋亡减弱,抗体聚集,抑制免疫应答的效应减低,导致对卵巢的免疫性损伤,卵巢功能的破坏、衰竭。补肾中药复方左归丸可通过调节Fas/FasL系统平衡,调节免疫反应及细胞毒性淋巴细胞功能,促进B细胞凋亡,抑制抗体聚集,减轻卵巢免疫炎性反应,改善卵巢功能。

Objective： To probe the effect of Zuogui pill （ZGP）, a Chinese compound recipe for tonifying Shen, on expression of Fas/ FasL Protein in ovaries of mice with immune premature ovarian failure （POF）. Methods：BALB/C female mice modal of POF was established by multiple sites subcutaneous iniection of zona pellucida 3 of mice, and treated with different dosage ZGP of low,middle and high one, with prednisone and diaethylstilbestrol as positive control. Detected expression of Fas/FasL protein in folliculus and stroma of murine ovaries by immunohistochemical method. Results：The expression of Fas protein in great folliculus and small ones recreased obviously in POF mice. The expression of FasL protein in small folliculus and stroma increased obviously in modal mice. The expression of Fas protein in great folliculus and small ones increased obviously in the mice of different dosage ZGP. The expression of Fas protein increased in stroma but the expression of FasL protein decreased in small folliculus in the mice of low dosage ZGP. The expression of Fas protein in stroma also increased obviously in the mice of high dosage ZGP. The differences were significant compared with the model mice. Conclusion： In POF mice the Fas/FasL system were unbalance. The apoptosis of B cells weaken. The antibody of anti-zona pellueida（AZpAb） assembled. The function of inhibiting immune response decreased. The ovaries were injured by immune inflammation and the ovarian function were destroy and failure. ZGP can improve the balance of Fas/FasL system, regulate immune response and functions of cytotoxic lymphocyte, promote the apoptosis of B cells, inhibite AZpAb assembled. ZGP can relieve ovarian immune inflammation and improve ovarian function.