纳米硒对小鼠移植瘤H_(22)的生长抑制作用及其机制

Anti-tumour effect of Nano Seleniums and its mechanism for mice with transplanted tumour H_(22)

目的:研究纳米硒对小鼠移植瘤H22的生长抑制作用及其作用机理,探讨纳米硒抗肿瘤治疗的可能性。方法:采用体内抗肿瘤法观察各组的抑瘤率;肿瘤组织切片HE染色、甲基绿-派洛宁染色、透射电镜观察其病理改变;免疫组化法检测Bax蛋白的表达水平;HE切片检测对胸腺脾脏的影响。结果:纳米硒抑制小鼠移植瘤H22的生长、浸润,减少核分裂,增加凋亡,血管生成减少;电镜见肿瘤细胞的典型凋亡形态,免疫组化显示肿瘤组织的Bax蛋白表达上调,SI、TI无明显降低,脾小体、脾巨噬细胞数升高。结论:一定剂量的纳米硒可能通过增强免疫作用和上调Bax蛋白的表达诱导肿瘤细胞凋亡两种主要途径来实现抗肿瘤作用。

Objective： Studying Nano Selenium＇s effect on growth-inhibition and mechanism for mice with transplanted tumor H22, discussing whether it is possible to treat turnout with Nano Selenium. Method： Observe the tumour-inhibitation rate of each group through anti-turnout experiment in the internal body; observe the lesions of turnout through HE staining, Methyl green-pyronine staining and electroscope observation; investigate the expression of Bax by immunohistochernistry; observe Nano Selenium＇ s influence on spleen and thymus through HE staining. Results： Nano Selenium can prevent transplanted tumor H22 from growing and infiltrating ef- fectively; The number of the nucleus fission and blood vessels decreased, The number of the apoptosis increased. Electroscope observation showed that tumor tissue had more apoptosis condition;immunohistochemistry showed that the expression of Bax protein was upregulated,thymus index （TI）and spleen index（SI） had no significant decrease among all experimental groups, but the number of macrophage and spenic segment increased in Nano Selenium group. Conclusion： The results suggest that a certain dose of Nano Selenium has obvious tumor inhibition through enhancing the immune system effects and Up-regulated expression of Bax protein in the internal body experimento