多胺类似物CHEN,CPEN和BENS对Hela宫颈癌细胞生长的影响

Effect of polyamine analogues,CHEN,CPEN and BENS,on the growth of Hela cervical cancer cells

目的:探讨多胺类似物CHEN,CPEN和BENS对宫颈癌细胞株Hela生长的影响。方法:MTT法检测细胞的生长状况;亚凋亡峰流式细胞分析法及DNA片段化分析法检测细胞凋亡;化学分析法测定精胺氧化酶(SMO)活性。结果:3种多胺类似物均可显著抑制Hela癌细胞增殖,抑制作用随药物浓度加大而增加,其细胞毒性大小依次为CHEN>CPEN>BENS。细胞凋亡分析发现,CHEN,CPEN和BENS可诱导Hela细胞程序性凋亡,导致凋亡峰出现和明显的DNA片段化。用10μmol.L-1CHEN,CPEN和BENS处理Hela细胞24 h可显著性抑制精胺氧化酶活性。结论:CHEN,CPEN和BENS通过诱导细胞凋亡而抑制Hela癌细胞生长,其诱导凋亡的作用与精胺氧化酶活性无关。

Objective：To study the effects of polyamine analogues, CHEN [ N^1-ethyl-N^11-（ cycloheptyl）- methyl-4,8, diazaundeeane ], CPEN [ N^1 -ethyl-N^11 -（ eyclopropyl）-methyl-4,8-diazaundeeane ] and BENS [ his （ethyl） -norspermine ] on the growth of Hela human cervical cancer cells. Methods ： MTT reduction was used to assay the cell proliferation. Sub-G1/flow cytometry and DNA fragmentation assays were used to determine the cell apoptosis. Chemical analysis method was used to determine the enzymatic activity of spermine oxidase. Results：All the polyamine analogues inhibited the growth of Hela cancer cells in a concentration-dependent manner with an order of cytotoxicity ： CHEN 〉 CPEN 〉 BENS. CHEN, CPEN and BENS induced apoptosis of Hela cells and resulted in appearance of sub-G1 peaks and DNA fragmentations. Significant decrease of spermine oxidase activities were observed after 24-h treatment with 10 μmol · L^-1 CHEN, CPEN and BENS. Conclusion： Polyamine analogues （ CHEN, CPEN and BENS） inhibit proliferation of the Hela cervical cancer cells by inducing apoptosis; the inhibition of apoptosis is not related to the activity of spermine oxidase.