摘要
目的：建立高效液相色谱-串联质谱法同时测定人血浆中曲马多和文拉法辛的浓度。方法：血浆样品经NaOH碱化后,用CH2C l2萃取;流动相为CH3OH-5 mmol.L^-1醋酸铵缓冲液（90∶10,10%甲酸调pH值为6.0）,流速：0.2mL·min^-1,采用电喷雾离子源（ESI）,以多反应离子监测（MRM）方式进行检测。结果：曲马多和文拉法辛的线性范围分别为6.250～1600和1.562～400.0μg.L^-1,最低定量限分别为6.250和1.562μg.L^-1。方法回收率分别为97.6%～103.8%和96.2%～102.1%,萃取回收率分别为55.1%～74.0%和50.4%～53.6%,日内和日间RSD均〈10%。结论：本法准确,灵敏,重现性好,可用于曲马多和文拉法辛血药浓度的检测和药动学等相关研究。
Objective：To establish a HPLC-MS/MS method for the determination of tramadol and venlafaxine in human plasma. Methods：Plasma samples were extracted With CH2Cl2. Mobile phase consisted of CH3OH-5 mmol·L^-1 ammonium acetate （90： 10,10% formic acid adjust the pH to 6.0） at the flow rate of 0.2 mL·min^-1 The samples were subsequently analyzed by mass spectrometry with an ESI source and then quantified by multiple reaction monitoring （MRM）. Results：The linear calibration curves of tramadol and venlafaxine were in the ranges of 6. 250 - 1 600 and 1. 562 - 400.0/μg· L^- 1, and the low determination limits were 6. 250 and 1. 562μg· L^- 1, respectively. The relative recoveries of tramadol and venlafaxine were 97.6% - 103.8% and 96.2% - 102.1% ,the extraction recoveries of tramadol and venlafaxine were 55.1% -74.0% and 50.4% -53.6% , and the within-day and between-day RSDs were less than 10% , respectively. Conclusion：This method is sensitive and reproducible, and the results are reliable and accurate.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2008年第6期498-501,505,共5页
Chinese Journal of New Drugs
