摘要
杜仲外植体在MS+2,4-D2.0mg/L的培养基上可诱导出生长速度快、离散性好、适合于悬浮培养的愈伤组织;愈伤组织在MS+2,4-D2.0mg/L+CH300mg/L的液体培养基上进行振荡培养,经3~4次继代,即可建立起悬浮细胞系;悬浮细胞的形状为长形、椭圆形、圆形和“月牙”形。
Many kinds of medium were used in the callus culture of Eucommia ulmoides Oliv., and the medium of MS+2,4-D 2.0 mg/L was proper to the growth of callus. The callus obtained were very loose, growth rapidly, and suitable for suspension culture. The callus were suspended in MS medium with 2,4-D 2.0 mg/L+CH 300 mg/L. After 3-4 generations of subculture, single cell suspension was obtained. The cells were long, ellipsoidal, round or other shapes.
出处
《河北大学学报(自然科学版)》
CAS
1997年第3期78-80,共3页
Journal of Hebei University(Natural Science Edition)
基金
河北省教委资助
关键词
杜仲
愈伤组织
悬浮培养
细胞培养
Eucommia ulmoides Oliv. Callus Suspension culture