摘要
目的:研究化浊行血汤对局灶性脑缺血再灌注大鼠行为学、脑梗死体积和细胞坏死、凋亡的影响。方法:用大脑中动脉线栓法建立大鼠局灶性脑缺血再灌注模型,检测脑梗死体积,并比较其神经功能行为积分;应用流式细胞仪和Annexin-V试剂盒检测缺血中心区和缺血半暗带区的细胞坏死率和凋亡率。结果:与模型组比较,治疗组脑梗死体积明显缩小(P<0.01),治疗组大鼠神经功能行为积分明显下降(P<0.01),缺血半暗带区的细胞坏死率和凋亡率明显降低(P<0.05)。结论:化浊行血汤可减少局灶性脑缺血再灌注大鼠脑梗死体积,改善脑缺血后神经行为症状,降低缺血半暗带区的细胞坏死率和凋亡率。
This study tries to observe the effects and molecular mechanism of Hua-zhuo-xing-xue decoction on treating ischemic cerebral injury. We created the middle cerebral artery occlusion （MCAO） rats to study the focal cerebral ischemic. In order to evaluate the therapeutic effects of Hua-zhuo-xing-xue decoction, we measured the changes of MCAO rats in nervous function impairment, cerebral infarction volume, apoptosis, and necrosis rate of brain cells. We found that Hua-zhuo-xing-xue decoction could significantly improve nerve function impairment in MCAO rats, reducing cerebral infarction volume, and slowing down the apoptosis in ischemic ambitus. We believe that Hua-zhuo-xing-xue decoction is desirable for treating ischemic cerebral lesion by protecting the never system.
出处
《世界科学技术-中医药现代化》
2008年第1期76-79,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
关键词
血浊
脑梗死
化浊行血汤
细胞凋亡
Hua-zhuo-xing-xue decoction
blood turbid
ischemic cerebral injury
