根霉2^#（Rhizopus sp.2^#）产酶条件研究

The Study of the Produced Condition of Rhizopus sp. 2 #

对影响根霉２＃（Ｒｈｉｚｏｐｕｓｓｐ．２）产生生淀粉分解酶的碳源、氮源、无机盐、温度、ｐＨ值等进行优化组合试验，摸索出了一个最佳的产酶条件，该条件为：木薯渣５％、花生麸０．７５％、尿素０．１％、Ｋ２ＨＰＯ４０．１％、ＭｇＳＯ４０．０７５％、ｐＨ值５．０、３２℃摇瓶培养９６ｈ。使用该条件可使得发酵酶液的酶活达到７．７５Ｕ／ｍＬ。

The effects of medium components such as carbon,nitrogen,inorganic salt and culture conditions such as pH,temperature on Rhizopus sp. 2 # were investigated. The optimum formative condition of the raw starch decomposing enzyme was the medium containing 5% cassava residue, 0 75% peanut meal, 0 1% urea,0 1% K 2HPO 4,0 075% MgSO 4·7H 2O and pH5 0. The raw starch decomposing enzyme activity of Rhizopus sp. 2 cultivated on this condition in 32℃ for 96h reached 7 75 U/mL.