植物药洪天肝康防治小鼠肝硬化的药效

Experimental study of herbalbased medicine Hong Tian Gan Kang on the intervention and therapy for hepatic cirrhosis

目的:研究植物药洪天肝康(HTGK)对小鼠实验性肝硬化的防治作用.方法:将ICR♀小鼠,随机分成正常对照组、模型组、预防组和治疗组.正常对照组:室温下常规饲养;模型组:ip 200 mL/L CCl_4;预防组:ip 200 mL/L CCl_4同时给予HTGK灌服;治疗组:造模d 180开始给予HTGK灌服,同时继续ip 200 mL/L CCl_4.模型组和预防组于60、90、180d:治疗组于30、60、90 d分别进行肝脏B超检测,并取出肝脏组织进行HE、VG染色和PAS反应,以观察其组织病理学的变化.应用免疫组织化学方法进行肝组织中转化生长因子-β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)的标记,以探讨HTGK的作用途径.TGF-β1和α-SMA的阳性标记物应用图像分析仪进行定量测定.结果:模型组肝脏呈重度变性及炎性细胞浸润,可见纤维条索形成;预防组较模型组肝纤维化程度轻;治疗组显示正常肝细胞数量增多,肝纤维化程度明显减轻.模型组TGF-β1、α-SMA阳性反应物与正常对照组相比显著增加(TGF-β1,60d:0.269 vs 0.155;90d:0.306 vs 0.155;180d;0.336 vs 0.160;α-SMA,60 d:0.269 vs 0.150;90d:0.299 vs 0.155;180d:0.322 vs 0.160,均P<0.01):治疗组TGF-β1、α-SMA阳性反应物与模型组相比显著降低(0.220,0.203,0.185 vs 0.336,P<0.01;0.245,0.211,0.185 vs 0.322,P<0.01).结论:植物药HTGK对肝硬化的形成具有积极的干预作用.

AIM：To investigate the prevention and treatment of Hong Tian Gan Kang（HTGK）on experimental hepatic cirrhosis. METHODS：The female ICR mice were allocated into traditional Chinese medicine（HTGK）prevention group,treatment group,animal model group,and control group.During the process of inducing hepatocirrhosis by CCl4 celiac injection in ICR mice,the HTGK group received HTGK per day,while the model group was created into the animal model by the above means without treatment.The mice of control group were normally raised without being created into models.The control group,animal model group and（HTGK）prevention group were sacrificed randomly at the end of the 60d,90d and 180d; while treatment group was sacrificed randomly at the end of the 30d,60d and 90d.Tissue specimens were taken.Animals received liver histopathology and ultrastructure test. RESULTS：Up to day 180,marked hepatic fatty changes,inflammation,necrosis and fibrosis were observed in mice of model group. In contrast,these alternations were attenuated by HTGK administration although mild fatty changes remained.Compared with the CCl4- induced cirrhotic mice,histological changes of fibrosis were improved significantly in the mice treated with HTGK.Liver histopathology showed that the contents of transforming growth factor-β1（TGF-β1）and alpha-smooth muscle actin（α-SMA）in the animal model group were significantly higher than those in the control group（TGF-β1,60d：0.269 vs 0.155;90d：0.306 vs 0.155;180d：0.336 vs 0.160;α-SMA,60d：0.269 vs 0.160;90d：0.299 vs 0.150;180d：0.322 vs 0.155, P〈0.01）.However,the contents of TGF-β1 andα-SMA in the therapy group were significantly lower than those in the model group（0.220,0.203, 0.185 vs 0.336,P〈0.01;0.2451,0.2113,0.185 vs 0.3217,P〈0.01）. CONCLUSION：HTGK has suppressive,preven- tive and curative effect on hepatic fibrosis and hepatocirrhosis.