白介素6基因启动子-572C/G多态性与非酒精性脂肪肝炎的关系

C/G homozygotes of interleukin-6 single nucleotide polymorphism at-572 are associated with nonalcoholic steatohepatitis

目的:探讨白介素6(IL-6)基因启动子-572C/G多态性与非酒精性脂肪肝炎(NASH)相关性.方法:应用聚合酶联反应-限制性片段长度多态性(PCR-RFLP)方法,分别检测NASH患者78例和正常者104例IL-6基因启动子-572C/G多态性的变异.采用ELISA法检测NASH患者组IL-6血清含量.结果:IL-6基因启动子-572位点的C等位基因频率高于健康对照组,两组基因分布频率有显著统计学差异(0.564 vs 0.404,P<0.05):NASH患者组中CC基因型携带者IL-6血清含量显著高于其他基因型携带者(6.54±4.21 vs 4.68±2.88,P<0.05),但HOMA-IR及BMI与其他两组基因型相比较并无显著统计学差异.结论:IL-6基因启动子-572位点的C等位基因与NASH发病具有一定程度相关性.

AIM：To investigate the relationship between the C/G genotypes of interleukin-6（IL-6）single nucleotide polymorphism（SNP）at 572 and the development of nonalcoholic steatohepatitis （NASH）. METHODS：Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to determine a common polymorphism of the human IL-6 gene-572C/G in 104 controls and NASH 78 patients.Enzyme linked immunosorbent assay（ELISA）was used to determine the concentration of serum IL-6 in 78 patients with NASH. RESULTS：Homozygotes for-572C of IL-6 gene were more frequent in NASH patients than in controls（0.564 vs 0.404,P〈0.05）.Level of IL-6 was significantly higher in NASH patients with IL-6-572C/C genotype than that in NASH patients with other genotypes（6.54±4.21 vs 4.68±2.88,P〈0.05）.The significant difference in HOMA-IR was not seen in three genotypes. CONCLUSION：The C/G homozygotes of IL-6 single nucleotide polymorphism at-572 have something to do with the pathogenesis of NASH.