激光诱导大鼠大肠早癌组织自体荧光光谱特征

Characteristics of laserinduced autofluorescence spectra in early colorectal carcinoma

目的:探索激光诱导大肠早癌组织自体荧光光谱特征.方法:采用二甲肼ip法建立SD大鼠大肠癌动物模型,对大鼠结肠和直肠可疑病变或肿块部位用370 nm激光照射,诱导组织产生自体荧光并收集光谱后取材,根据病理诊断分为正常组、早期癌组、进展期癌组,分析各组光谱特征.结果:正常64例,早期癌78例,进展期癌84例,共226例.各组光谱在460 nm和505 nm处附近有波峰;正常组460 nm左右波峰对应的波长与早期癌组、进展期癌组对应波长比较差异有显著性(457.66±3.28 vs 467.87±7.71,468.60±4.53,P<0.05).73%(61/84)的进展期大肠癌组织、69%(54/78)的早期大肠癌组织荧光光谱在635nm附近有荧光峰,而正常组织缺乏该峰.早期癌组和进展期癌组自体荧光在460 nm处的荧光强度比在正常组相应强度弱.早期癌组和进展期癌组I_(635)/I_(460)(1.9507±1.1460,2.1368±1.4721)和I_(635)/I_(600)值(0.4215±0.2582,0.4482±0.2309)分别较正常组I_(635)/I_(460)和I_(635)/I_(600)值大且差异有显著性(0.7494±0.1077,0.1416±0.0439,P<0.05).结论:激光诱导的大鼠大肠正常组织与大肠癌组织(包括早期和进展期大肠癌)自体荧光光谱之间存在差异,自体荧光光谱部分特征可用于大肠癌早期诊断.

AIM：To analyze the characteristics of laser-induced autofluorescence spectra in early colorectal carcinoma. METHODS：An animal model of colorectal carcinoma was established by injecting DMH in abdominal cavity of Sprague-Dawley rats.Samples were collected from normal,early-stage and advanced carcinoma tissues,respectively.The characteristics of their laser-induced autofluorescence spectra were analyzed. RESULTS：A total of 226 samples were collected,including 64 normal colorectal tissue samples,78 early-stage and 84 advanced colorectal carcinoma tissue samples.Spectra peaks were observed at about 460 nm and 505 nm in different groups.The wavelength at 460 nm in normal tissue samples differed significantly from those in early stage and advanced colorectal cancer tissue samples（457.66±3.28 vs 467.87±7.71,468.60±4.53,P〈0.05）.Spectra peaks were found at 635 nm in 73% advanced colorectal carcinoma tissue samples and 69% early-stage colorectal carcinoma tissue samples,but not in normal colorectal tissue samples.The mean wavelength at 460 nm was longer in normal colorectal tissue samples than in early stage and advanced colorectal carcinoma tissue samples（457.66±3.28 vs 467.87±7.71,468.60±4.53,P〈0.05）.The values of I635/I460and I635/I600in early-stage（1.9507±1.1460,0.4215±0.2582）and advanced carcinoma tissue samples（2.1368±1.4721,0.4482±0.2309） were higher than those in normal colorectal tissue samples（0.7494±0.1077,0.1416±0.0439,P〈0.05）. CONCLUSION：The characteristics of laser- induced autofluorescence spectra are different in normal colorectal tissue,early-stage and advanced colorectal carcinoma tissue,and can be used to diagnose early-stage colorectal carcinoma.