肝内胆管癌细胞系ICC-9810与肝细胞系L02蛋白质组学的差异分析

Differential expression of proteins in intrahepatic cholangiocarcinoma cell line ICC-9810 and liver cell line L02

目的:分析肝内胆管癌细胞系ICC-9810与正常肝细胞系L02的蛋白质表达差异,筛选肝内胆管癌的潜在分子标志物.方法:应用表面增强激光解吸离子化(surface enhanced laser desorption/ionization,SELDI)蛋白质芯片技术检测肝内胆管癌及正常肝细胞系的蛋白质谱.用PBSII-C型蛋白质芯片阅读机读取数据,采用Proteinchip Software3.0.2软件分析数据.结果:IMAC3、WCX2两种蛋白芯片共捕获376个蛋白峰,发现27个差异蛋白.与正常肝细胞系L02蛋白谱相比,9个蛋白在肝内胆管癌细胞系ICC-9810中高表达,18个蛋白在肝内胆管癌细胞系ICC-9810中低表达.其中3767、7999、10555、12163、22066和26794Da6个差异蛋白可被WCX2和IMAC3芯片共同捕获.结论:肝内胆管癌与正常肝细胞存在差异蛋白表达,这些差异蛋白为寻找肝肿瘤标志物,了解肝内胆管癌的发病机制提供了重要线索。

AIM： To analyze the differential expression of proteins in intrahepatic cholangio-carcinoma cell line ICC-9810 and normal liver cell line L02, and to screen the potential molecular markers for the diagnosis of intrahepatic cholangiocarcinoma. METHODS： Surface enhanced laser desorption / ionization （SELDI） mass spectrometry with ProteinChip IMAC3 and WCX2 was performed to compare the expressed proteins in intrahepatic cholangiocarcinoma cell line ICC-9810 and norreal liver cell line L02. Protein profiling was examined with a PBSII-C ProteinChip reader and proteome data were analyzed using Proteinchip Software 3.0.2. RESULTS： ProteinChip IMAC3 and WCX2 captured all the 376 protein peaks in ICC-9810 and L02 cell lines. Among the identified 27 differential proteins in ICC-9810 cell line, 9 showed a higher expression and 18 a lower expression as compared with L02 cell line. Six proteins, namely 3767, 7999, 10 555, 12 163, 22 066 and 26 794 Da, were conjointly detected by IMAC3 and WCX2 ProteinChip. CONCLUSION： Expression of proteins is different in intrahepatic cholangiocarcinoma cells and normal liver cells. These differentially expressed proteins can be used as candidate biomarkers of intrahepatic cholangiocarcinoma and provide insights into the pathogenesis of intrahepatic cholangiocarcinoma.