康盆炎胶囊中小檗碱的含量测定

Determination of Berberin in Kangpenyan Capsule

目的：建立反相高效液相法测定康盆炎胶囊中小檗碱含量的方法。方法：采用烷基硅烷键合硅胶柱，流动相为甲醇-乙腈-水（40：21：39）（内含0．8g十二烷基磺酸钠和0．5g酒石酸）；检测波长为346nm。结果：小檗碱在（0．083～0．747）μg范围内呈良好的线性关系；平均回收率为96．34％，RSD为0．98％。结论：该方法不仅操作简单、准确，且重复性好，可用于康盆炎胶囊的质量控制。

Objective： To develop a RP-HPLC method for determination of berberin in Kangpenyan Jiaonang. Methods： Berberin was separated completely on an C18 column, using a mixture of methanol, acetonitrile and water（40：21：39） （containing 0.8 g Sodium dodecyl sulphonate and 0.5 g tartaric acid） as mobile phase, with UV detection at 346 nm. Results：The linear range of berberin was in （0.083-0.747）μg, the recovery rate was 96.34%, RSD=0.98% . Conclusions：The method is very stable, accurate, and repeatable. It can be used for quality control of Kangpenyan Jiaonang.