柴荆注射液中柴胡气相色谱指纹图谱研究

Gas Chromatographic Fingerprint for Bupleurum chinese DC.of Chaijing Injection

目的:应用气相色谱(GC)法建立柴荆注射液中柴胡挥发油的指纹图谱。方法:收集10批北京产药材柴胡代表性样品,按生产工艺制备柴胡水蒸汽蒸馏液,正己烷提取后,用Varian CP-Sil 24 CB毛细管柱,程序升温分离,FID检测。结果:方法有良好的重复性、精密度和稳定性(RSD<3%,n=6),建立的北京柴胡药材指纹图谱确定9个共有峰,用中南大学版相似度评价软件计算,相关系数大于0.9。结论:建立的指纹图谱可为柴荆注射液的质量控制提供依据。

Objective： To establish GC fingerprint for quality control of volatile oil in Bupleurum chinese DC.of Chaijing Injection. Methods： A sum of 10 batches of Bupleurum Chinese DC. samples were collected and identified. The GC equipped with FID detector, and a Varian CP-Sil 24 CB capillary column was used. The temperature program of column oven was as follows ： 50 ℃, held for 3 min ; increased at a rate of 2 ℃ · min^- 1 to 110 ℃, then at 6 ℃ · min^- 1 to 250 ℃, held for 5 min. Results： The method had good precision, repeatability and stability （RSD 〈 3%, n = 6）. The fingerprints from the 10 samples were well-uniformized and the correlative coefficient was over 0.9. Conclution： The fingerprint is reliable and can be used for quality control of volatile oil in Chaijing Injection.