摘要
目的:观察加味四妙汤对微晶型尿酸钠(MSU)诱导的兔急性痛风性关节炎的防治作用,并初步探讨其作用机制。方法:用MSU诱导兔痛风性关节炎,致炎5 h后收集关节液进行白细胞计数,测定关节液中细胞因子和炎症因子含量,并对关节及周围软组织进行病理组织学检查。结果:加味四妙汤12,6 g.kg-1灌胃可显著降低兔关节液白细胞数以及肿瘤坏死因子-α(TNF-α),前列腺素E2(PGE2),IL-1β,IL-8水平,减轻关节及软组织水肿和炎细胞浸润、变性坏死。加味四妙汤各组对关节液中IL-6,一氧化氮(NO)抑制作用不明显。结论:加味四妙汤能明显改善MSU诱导的兔痛风性关节炎,其机制可能是抑制炎症细胞趋化和激活,抑制炎症因子、细胞因子的合成与释放。
Objective：To explore the effects and mechanisms of Si-miao Decoction on gouty arthritis induced by MSU in rabbits. Methods： Si-miao Decoction and colchicine were orally administrated for 7 or 4 days. Acute gouty arthritis model of rabbits was induced by MSU suspension intraarticular injection. After MSU injection for 5 hours, synovia of each rabbit was collected to do leukocyte count. Meanwhile, relevant cytokines levels in synovia of each group were measured. Histopathologic examination of knee joint and surrounding soft tissue of each rabbit was also performed. Results： Si-miao Decoction 12 g· kg^- 1 and 6 g· kg^- 1 could significantly decrease the leukocyte quantity and the levels of TNF-α, PGE2, IL- 1β and IL-8 in synovia of model rabbits, as well as ameliorating edema and inflammatory cell and inflammatory cell infiltration in knee joint of model rabbits. Conclusions： Si-maio Decoction could obviously alleviate symptoms of acute gouty arthritis induced by MSU in rabbits. Mechanisms may lie in inhibiting leukocyte activation, chemotaxis, and decreasing cytokines levels in synovia.
出处
《中国实验方剂学杂志》
CAS
2008年第2期42-45,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
安徽省教育厅自然基金资助(No.99JLO125)
