大黄对糖尿病大鼠血管病变保护机制的实验研究

Vascular Protective Effects of Da Huang in Diabetes Mellitus Rats

目的:探讨大黄对糖尿病血管病变的保护机制。方法:制备糖尿病大鼠模型,并给予大黄干预,8周后,取血测定一氧化氮(NO)及内皮素-1(ET-1)。取胸主动脉环观察不同累积浓度的乙酰胆碱(Ach)对去甲肾上腺素(NE)引起的血管收缩的抑制率。另留取一段胸主动脉制备病理切片,SP法免疫组化染色,观察细胞间黏附分子-1(ICAM-1)及血管细胞间黏附分子-1(VCAM-1)的阳性表达。结果:大黄组与模型组相比能明显抑制升高的血浆ET-1(P<0.05);升高NO的水平(P<0.05);但均达不到正常组的水平(P<0.05)。模型组血管环对NE的收缩反应明显强于正常对照组及大黄组(P<0.05),且大黄组对NE的收缩反应亦强于正常对照组(P<0.05)。在相同Ach浓度下,模型组及大黄组胸主动脉环对Ach的舒张作用明显弱于正常组(P<0.05),而大黄组强于模型组(P<0.05)。大黄组能明显抑制糖尿病大鼠胸主动脉ICAM-1及VCAM-1表达(P<0.05)。结论:大黄具有降低大鼠ET-1及升高NO的作用,能够保护糖尿病大鼠内皮依赖的血管舒张功能,且能够抑制ICAM-1及VCAM-1的表达,具有抗动脉硬化作用。

Objective：To explore the protective mechanism of Chinese Herb-Da Huang on the vascular disease in diabetes mellitus （DM） rats. Methods： The DM rat model was made. Da Huang was given to treatment group. 8 weeks later,blood sample was taken to test nitric oxide（NO） and endothelin-1（ET-1 ）.Thoracic aortic rings to observe the ratio of different concentration of Ach inhibite constraction caused by NE. Took down another part of aorta to make pathology slice,method of SP immunohistochemistry staining, observed the expression of ICAM-1 and VCAM-1. Result： Da Huang group obviously decreased plasma ET-1 and increased the level of NO comparied with Diabetes group （P 〈 0.05）, but could not reach the level of the normal control group （P 〈 0.05）. The expression of ICAM-1 and VCAM-1 could be obviously inhibited in Da Huang group comparied with diabetes contrast group. But obviously stronger than normal control group （P 〈 0.05）. Conclusion： Chinses medicine Da Huang could decrease the level of ET-1 and increase the level of NO in diabetes rats, they have the function of protect the endothelium of vessel, and could inhibte the expression of ICAM-1 and VCAM-1, act as the role of anti-atherosclerosis.