黄连须药材的定性与定量分析

Qualitative and Quantitative Analysis of Radix Copidis

目的探讨检测黄连须质量的方法。方法本实验采用显微鉴别及薄层色谱法对黄连须进行定性分析,采用HPLC法测定不同产地的10批黄连须的含量。结果黄连须中盐酸小檗碱的含量为0.73%～1.82%（g/g）;加样平均回收率为99.2%,RSD=1.7%（n=6）;回归方程为y=73432x-1162.9,R=0.9999,线性范围是0.06～0.60μg。结论该方法可作为黄连须的定性与定量分析方法。

objective To investigate the detective method for the quality standard of Radix Copidis. Methods Microscopic identification and TLC were used for the qualitative analysis of Radix Copidis. The content of 10 groups from different producing areas was assayed by HPLC. Results The content of Berberine Hydrochloride in Radix Copidis was 0. 73% - 1.82% （g/g） ; the average recovery was 99.2% , and RSD = 1.7% （n = 6） ; the regression equation was y = 73432x - 1162.9, with correlation coefficient R = 0. 9999, and linear range was between 0. 06 - 0. 6 μg. Conclusion This method is simple and accurate. It can be used for the qualitative and quantitative analysis of Radix Copidis.