木薯细菌性萎蔫病菌的检疫方法研究

Method for inspection and identification of Xanthomonas campestris pv.manihotis.

本文对木薯细菌性萎蔫病菌的致病性测定、细菌的分离、细菌的培养条件和培养基选择、细菌的生理生化测定、分子生物学鉴定方法等方面进行了系统研究,确定了该病菌的菌落鉴定特征,建立了从木薯繁殖材料上进行病原菌检测的快速、灵敏、准确的PCR检测方法。

This subject did a systematic research on the cassava bacterial blight, caused by Xanthomonas campestris pv. manihotis , including its pathogenicity, isolation cultural condition, the choice of the medium, physiological and biochemical characteristics, and molecular biology identification etc. We confirmed the bacterial colony identification characteristics, and established a rapid, sensitive and accurate PCR method to detect Xanthomonas campestris pv. manihotis .