摘要
根据植物NBS类抗病基因保守氨基酸序列P-loop和疏水氨基酸GLPL保守序列设计简并引物,从香蕉抗镰刀菌枯萎病(4号小种)材料IN21(Musa spp.cv.'IN21',AA)和Rose(M.spp.cv.'rose',AAA)的基因组DNA中扩增获得4个RGA,四条DNA片段长度分别为527 bp、537 bp、534 bp和547 bp,分别命名为"RGA-IN1"、"RGA-IN2"、"RGA-Rose1"和"RGA-Rose2"(GenBank Accession:EF488469、EF488470、EF488471和EF488472);同源性分析表明,均与已报道的植物抗病基因有不同程度的同源性,具有P-loop、Kinase-2、RNBS-B(Kinase-3a)以及GLPL等保守氨基酸序列,属于non-TIR-NBS类候选抗病基因。
Degenerate primers, designed based on the consensus amino acid sequence of plant resistance genes, such as NBS motifs e.g. P-loop and hydrophobic amino acid sequences e.g. GLPL, were used to amplify the genomic DNA of banana germplasms that would be resistant tofusarium wilt. Four DNA fragments with length of 527 bp, 537 bp, 534 bp and 547 bp were obtained and sequenced, named as ＂RGA-IN1 ＂, ＂RGA-IN2＂, ＂RGA-Rosel ＂, ＂RGA-Rose2＂. Blast analysis indicated that the obtained sequences had different homologies to the plant disease resistant genes in conservative motifs, such as P-loop, Kinase-2, RNBS-B （Kinase-3a） and GLPL, and these sequences would be assigned to a non-TIR-NBS group.
出处
《分子植物育种》
CAS
CSCD
2007年第F11期57-60,共4页
Molecular Plant Breeding
基金
广东省农业科学院博士启动项目(05-博-01)
