弥漫性脑损伤模型大鼠细胞因子在额叶表达及血清含量变化

Change in the expression of interleukin-6 and tumor necrosis factor-α mRNA in frontal lobe and in protein level in serum in diffused traumatic brain injury rat

目的:研究白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)在弥漫性脑损伤大鼠额叶的表达和血浆含量的变化。方法:采用Mamarou的弥漫性脑损伤的动物模型,分别于伤后不同时间收集额叶脑组织和静脉血,分别以RT-PCR法和放免法测定脑组织中IL-6和TNF-α的mRNA和血清中IL-6和TNF-α的含量变化。结果:在弥漫性脑损伤的额叶脑组织中IL-6 mRNA的表达较对照组明显升高,并在损伤后6h达到高峰;血清中IL-6的表达也较对照组升高,在12h达到高峰。而TNF-α mRNA的表达在12h明显高于对照组,24h达到高峰;血清中的TNF-α水平在24h内逐渐升高。结论:在弥漫性脑损伤中,IL-6和TNF-α在脑组织和血清中升高,在疾病的发展中起到重要的作用。

Objective：To investigate the change in the expression of interleIukin-6（IL-6） and tumor necrosis factor-α（TNF-α）mRNA in frontal lobe and serum IL-6 and TNF-α level in diffused traumatic brain injury rat model.Methods：Mamarou rat model of diffused traumatic brain injury was used in the study.Venous blood and frontal lobe were collected at different time after injury.The change of IL-6 and TNF-α mRNA expression and serum IL-6 and TNF-α level were detected with RT-PCR method and radioimmunoassay,respectively.Results：it was found that IL-6 mRNA level increased in diffused traumatic brain injury compared to the control group and reached to the peak 6 hours after injury.Serum IL-6 level also elevated in diffused traumatic injury rat compared to the control group and reached to the peak 12 hours after injury.TNF-α mRNA expression was up-regulated compared to the control group and reached to the peak 12 hours after injury.Serum TNF-α level elevated gradually within 24 hours after injury.Conclusion：Diffused traumatic brain injury induces upregulation of IL-6 and TNF-α mRNA expression in frontal lobe and elevation of serum level of them.IL-6 and TNF-αmay play an important role in the progress of diffused traumatic brain injury.