雌激素受体β基因多态性与原因不明月经过少的关系

The relationship between polymorphism of the estrogen receptor beta gene and unknown aetiology hypomenorrhea

目的:探讨西南地区雌激素受体β(ER-β)基因多态性与原因不明月经过少的关系。方法:选择西南地区100名原因不明月经过少患者为实验组,100名正常月经者作为对照组。应用分子生物学的方法研究ER-β基因RsaI和AluI限制性片段长度多态性和5号内含子的高变区CA重复序列多态性在实验组与对照组中分布。结果:RsaI和AluI限制性片段长度多态性在两组中均呈多态性分布。分离出9种CA重复序列等位基因,正常组和实验组的等位基因的分布频率差异无显著性。以重复次数n≤20作为SS型,n>20为LL型,再比较两组病人SS型和LL型等位基因的分布频率,差异有显著性。结论:ER-β基因多态性与原因不明月经过少有关,R等位基因可能是其保护因素,SS型等位基因可能是原因不明月经过少的危险因素。

Objective：To study the relationship between estrogen receptor βgene polymorphism and unknown aetiological hypomenorrhea in Southwestern China.Methods：100 eumenorrhea women were selected as the control group and another 100 hypomenorrhea patients as the experimental group from Southwestern China.Molecular biology was used to analyze restriction fragment length polymorphism（RFLP） of the RsaI and AluI and CA repeat polymorphism in 5 intron region in ERβgene.Then the ERβgene polymorphism genetype distribution was studied in experimental group and the control group.Results：RFLP of RsaI and AluI in the 2 groups were distributed with polymorphism.9 CA repeat sequence alleles were obtained.There was no difference in distributing frequencies of CA repeat polymorphism between the control and the experimental groups.SS genotype was defined as repeat number n≤ 20,LL for n〉20,which were significantly different in two groups.Conclusion：ERβgene polymorphism has a relation with unknown aetiological hypomenorrhea.R allele may be the protective factor,while SS allele may be its risk factor.