不同种源药用菊花、野菊和菊花脑的ISSR分子标记及遗传关系分析

Analyses of ISSR molecular marker and genetic relationship of different provenances of Dendranthema morifolium,D.indicum and D.nankingese

采用ISSR分子标记技术对来源于不同地区的19份药用菊花(Dendranthe mamorifolium Ramat.)种源、4份野菊(D.indicum L.)种源、1份菊花脑(D.nankingese Hand.-Mazz.)种源和1份杂交菊花‘黄金菊’(D.indicum×D.morifolium‘Gongju’)种源进行了遗传关系分析。从38条引物中筛选出6条引物,共扩增出66条带,平均多态性条带百分率达95.5%。聚类分析结果表明,取λ=16,25份种源可分成2大组,即野菊、菊花脑和杂交菊花归为一组,19份药用菊花种源归为一组;19份药用菊花种源又可根据原产地进一步分成2组,大部分原产北方的药用菊花种源的遗传关系较近,而大部分南方栽培的药用菊花种源也有相对较近的遗传关系。

Analysis of genetic relationships among nineteen provenances of Dendranthema morifolium Ramat., four provenances of D. indicum L., one provenance of D. nankingese Hand. -Mazz. and one hybrid provenance of D. indicum x D. morifolium ＇ Gongju＇ from different locations was carried out by using ISSR molecular marker technique. The results showed that six primers of ISSR were selected from thirty-eight primers and sixty-six bands were amplified by the six primers. Average percentage of polymorphic band was 95.5%. The results of cluster analysis indicated that twenty-five provenances could be divided into two groups at λ = 16, one group including D. indicum, D. nankingese and a hybrid, another group including nineteen provenances of D. morifolium, the latter was further divided into two groups according to geographic origins. provenances from the Northern of China were close provenances from the Southern of China were also Genetic relationships among most of D. morifolium and genetic relationships among most of D. morifolium close.