大孔吸附树脂柱层析法对西双版纳产刺五加总皂苷吸附性能的研究

Research on the Ability of Absorption and Disabsorption of the Total Glycosides from the Extracts of Xishuangbanna's Acanthopanax Senticosus by Appling Macroporus Resin

目的:研究HPD100型大孔吸附树脂分离富集西双版纳产刺五加中总皂苷的性能。方法:采用紫外分光光度法测定西双版纳产刺五加总皂苷的含量;用柱层析法研究HPD100大孔吸附树脂柱对刺五加总皂苷的吸附性能以及洗脱参数。结果:HPD100大孔吸附树脂对刺五加总皂苷静态比吸附量达109.8mg/g,静态解吸率为88.9%,动态比吸附量达48.87mg/g,50%乙醇的解吸率为78.51%.结论:HPD100型大孔吸附树脂可用于刺五加总皂苷的分离富集。

Objective： to study the separation effect of HPD100 Macroporus resin for the purification of Acanthopanax senticosus. Methods： UV spectrometric method was used to analyze the contents of the saponins. The adsorption and disabsorption of the saponins were also determined by the macroporus resin. Results： the ratio absorption of the the saponins is about 48.87mg/g and the disabsorption ratio is 78.51% when elucidated with the 50% alcohol. Conclusion： HPD100 resin can be used to refine the saponins of Acanthopanax senticosus,