不同陈化时期烤烟K326·云烟85绿原酸含量与褐变关系的研究

Research on Correlation between Chlorogenic Acid Content and Browning in Flue-cured Tobaccos K326 and Yunyan 85 in Different Aging Periods

[目的]测定不同陈化时期烤烟中绿原酸含量和颜色变化,探讨它们之间的相互关系。[方法]分别用高效液相色谱测定陈化36个月内烤烟K326和云烟85绿原酸含量变化,用比色仪测定烟叶颜色的变化,通过比较探讨绿原酸与褐变之间的关系。[结果]烤烟K326和云烟85在陈化的0-12个月内绿原酸的含量变化与颜色变化有明显的相关性,R2分别为0.993 5和0.983 3,表明在烟叶陈化初期褐变主要由酚类物质氧化所导致;在陈化的12-36个月时,绿原酸的含量变化与颜色变化基本不相关,R2分别为0.116 9和0.009 0,说明在此期间引起颜色变化的内因已不是酚类物质的氧化反应。[结论]在烟叶陈化的前12个月主要控制酚类物质的氧化程度可防止烟叶陈化初期褐变。

[Objective] The study aimed to determine the chlorogenic acid content and color change in flue-cured tobacco in different aging periods and discuss their correlation.[Method] The content changes of chlorogenic acid in flue-cured tobaccos K326 and Yunyan 85 in the aging period of 36 months were determined by HPLC and the color changes of tobacco leaves were determined by colorimeter.The correlation between chlorogenic acid and browning was discussed through comparison.[Result] The content change of chlorogenic acid had obvious correlation with color change in flue-cured tobaccos K326 and Yunyan 85 in the aging period of 0～12 months and the R2s were 0.993 5 and 0.983 3 resp.,showing that in the initial aging period of tobacco leaf,the browning was mainly caused by the oxidation of phenolic compounds.In the aging period of 12～36 months,the content change of chlorogenic acid didn＇t correlated with color change basically and the R2s were 0.116 9 and 0.009 0 resp.,indicating that in this period,the interior factor causing color to change was not the oxidation reaction of phenolic compounds.[Conclusion] In the first 12 months of the aging period of tobacco leaf,the browning in the initial aging period of tobacco leaf could be prevented mainly by controlling the oxidation degree of phenolic compounds.