亚精胺对黄瓜幼苗灰霉病的诱抗作用

Roles of spermidine in induced resistance of cucumber seedlings to Botrytis cinerea Pers.

采用根际基质注射结合叶面喷洒不同浓度亚精胺(Spd)的方法,测定了灰霉病菌侵染下黄瓜幼苗植株病情指数、防御酶活性和抗病相关物质含量等生理生化指标,以明确Spd对黄瓜幼苗抗灰霉病的诱导作用.结果表明:Spd处理黄瓜灰霉病病情指数比对照最高降低25.4%;苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和多酚氧化酶(PPO)活性最高分别比对照提高48.4%、635.9%和38.9%;总酚、类黄酮和木质素含量也明显增加.表明Spd可诱导黄瓜幼苗对灰霉病的抗性,且最佳处理浓度为150 mg.L-1.

Under the conditions of root substrate injection and foliar spray of different concentration spermidine, this paper measured the disease index, defense enzyme activities, and contents of disease resistance-related substances in cucumber （ Cucumis sativus L. ） seedlings infected by Botrytis cinerea Pers. , aimed to investigate the roles of spermidine in induced resistance of cucumber seedlings to B. cinerea. The results showed that after treated with spermidine, the disease index decreased by 25.4% , phenylalanine ammonia lyase （PAL） , peroxidase （POD） and polyphenol oxidase （PPO） activities increased by 48.4%, 635.9% and 38.9%, respectively, and total polyphenol, flavonoid and lignin contents increased significantly, compared with the control. All of these suggested that spermidine could induce the disease resistance of cucumber seedlings, and its optimum concentration was 150 mg·L^-1.