摘要
构建捻转血矛线虫Hc38保守结构域原核表达重组质粒pPROEXHTa-Hc38,在DH5a中经诱导表达约17ku的蛋白,表达产物通过Western blot鉴定,然后将其产物纯化,复性后制备油佐剂疫苗免疫4月龄绵羊,结果表明:对照组与实验组比较,虽然雌虫数量未见减少,但两者荷虫量差异显著(P<0.05)。
The recombinant plasmid pPROEXHTa-Hc38 expressing Haemonchus contortus Hc38 conservative domain was constructed. About 17 ku fusion protein was expressed in E.coli DH5a following IPTG induction. The recombinant protein was purified, renatured and prepared as oil adjuvant vaccine. The 4-mouth-old sheep were immunized with the adjuvant vaccine. The results showed that the number of mature Haemonchus contortus was markedly reduced （p 〈0.05） in comparison to the control group, although the number of female was not reduced.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2008年第4期295-299,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
新疆兵团科技局社会发展项目(2006GG31)
