奶山羊隐性乳房炎模型的建立及早期诊断指标的研究

Establishment of chronic mastitis model in milk goat

为选择和建立奶山羊隐性乳房炎的早期诊断指标,人工感染金黄色葡萄球菌并成功建立隐性乳房炎模型,检测了21d内奶山羊感染乳区乳中体细胞数和乳中的细菌浓度,模型奶山羊血液和乳中血清淀粉样蛋白A和结合珠蛋白的变化。同时做了各指标间的相关性分析。发现血清淀粉样蛋白A和结合珠蛋白在感染后血清中的浓度较感染前急剧升高,乳中血清淀粉样蛋白A和结合珠蛋白较感染前显著升高,各项指标呈显著正相关。结论为乳中血清淀粉样蛋白A和结合珠蛋白均可作为乳房金黄色葡萄球菌感染的敏感指标,且结合珠蛋白比血清淀粉样蛋白A更敏感。

In order to establish the early diagnosis indexes for chronic mastitis in milk goats, udder quarters of milk goats were inoculated with Staph aureus. Somatic cells （SC） and bacteria in infected milk samples were counted for 21 days. The concentrations of Serum amyloid＇protein A （SAA） and haptoglobin （Hp） in serum and whey samples were also tested. Correlations between the concentrations of bacteria and SC, SAA and SC, Hp and SC fxom milk samples were analysed, respectively. The concentrations of SAA and Hp in serum and whey samples were significantly higher than that of pre-infection samples. We concluded that SAA and Hp in milk samples could be used as sensitive parameters for diagnosis of mastiffs caused by Staphylococcus aureus.