菌酶共降解玉米秸秆的工艺研究

Technology for co-degradation of corn stalk by microorganism and enzyme

该文研究玉米秸秆菌酶共降解工艺,利用黄孢原毛平革菌固体发酵去除部分木质素,再添加外源纤维素酶、木聚糖酶降解纤维素和半纤维素。黄孢原毛平革菌培养12 d时木素过氧化物酶(LiP)酶活达到最大值11.3 u/g,15d时漆酶(Lac)酶活达到最大值0.0992 u/g,秸秆降解集中在10～20 d,第25d时菌解结束,干基总损失率为18.94%;再经过6 d的酶解,纤维素、半纤维素的相对含量分别从27.1%、20.3%下降到13.1%、11.9%。玉米秸秆经菌酶共降解,木质素、纤维素、半纤维素的降解率分别达到67.0%、60.4%、33.0%,每克秸秆还原糖含量达0.507 g。结果表明,菌酶共降解为玉米秸秆的生物利用提供了一种新方法。

The co-degradation technology of corn stalk by microorganism and enzyme was studied. Most of lignin was degraded though solid fermentation by Phanerochaete chrysosporium. Cellulose and hemicellulose were degraded through adding foreign cellulase and xylanase. Results show that maximum activities of LiP and Lac reach 11.3 u/g at the 12th day and 0.0992 u/g at the 15th day, rapidly degraded during 10-20 d, and the total loss rate of dry corn stalk is 18.94% after cultivation for 25d. The relative contents of celluloses and hemicellulose are decreased from 27.1% to 13.1% and from 20.3% to 11.9%, respectively after enzymolysis for 6 d. The degradation rates of lignin, cellulose and hemicellulose are 67.0%, 64.0% and 33%, the content of reduing sugar reaches 0.507 g/g （corn stalk） after co-degrading by microorganism and enzyme. Co-degradation technology by microorganism and enzyme provides a new method for utilization of corn stalk.