血管内皮生长因子受体-1对肝癌侵袭转移的影响

Effects of vascular endothelial growth factor receptor-1 on the invasion and metastasis of hepatocellular carcinoma

目的通过检测血管内皮生长因子受体-1（vascular endothelial growth factor receptor-1，VEGFR-1）在肝癌组织中的表达，探讨VEGFR．1在肝癌侵袭、转移中的作用。方法共取82例肝癌组织和癌旁肝组织的石蜡标本，采用免疫组织化学方法检测VEGFR-1、上皮标志物E-钙黏蛋白和间叶标志物波形蛋白的表达情况，并分析其关系。对肝癌患者临床病理学指标和HBV感染中VEGFR-1的表达进行分析。结果癌旁肝组织中无VEGFR-1表达，肝癌组织中VEGFR-1的表达率为89％（73／82）。VEGFR-1的表达在肿瘤分期、门静脉癌栓、肿瘤包膜、肿瘤直径、分化程度差异有统计学意义（X^2=22．192，15．934，16．751，20．154，6．487，P〈0．05）；对术后1、2年的复发率和术后2年生存率有影响（X^2=0．983，0．958，0．847，P〈0．05）；对术后1年的生存率无影响（X^2=0．359，P〉0．05）。VEGFR-1与E-钙黏蛋白的表达呈负相关（r=0．765，P〈0．01），而与波形蛋白的表达呈正相关（r=1．469，P〉0．05）。结论VEGFR-1可能在肝癌的侵袭、转移中发挥作用，其促肝癌侵袭转移可能与肝癌细胞发生上皮-间叶样表型转化有关。

Objective To investigate the effects of vascular endothelial growth factor receptor-1 （VEGFR-1） in the invasion and metastasis of hepatecellular carcinoma （HCC） by detecting the expression of VEGFR-1 in HCC tissues. Methods Paraffin-embedded tissue which containing HCC tissues and adjacent tissues were prepared from patients （ n =82） with HCC, then were analyzed by immunohistechemical technique for the expression of VEGFR-1, epithelial marker E-cadherin and mesenchymal marker Vimentin. The relationship between the expression of VEGFR-1 and pathological parameters, and the correlation between VEGFR-1 and E-cadherin, VEGFR-1 and Vimentin were analyzed. Results The expression rates of VEGFR-1 in HCC tissues and adjacent tissues were 89% （73/82） and 0, respectively. The difference of VEGFR-1 expression in portal vein tumor thrombus, tumor capsule, stage, size and differentiation grade of tumor had statistical significance （X^2 = 22. 192,15. 934, 16. 751,20. 154, 6. 487, P 〈 0.05）. The expression of VEGFR-1 had effect on the 1-, 2-year recurrence rate and 2-year survival rate （X2 =0.983, 0.958, 0.847, P 〈0.05）, but not on 1-year survival rate （X2 =0.359, P 〉0.05）. The expression of VEGFR-1 was negatively correlated with that of E-cadherin （ r =0.765, P 〈0.01） but positively with that of Vimentin （ r = 1.469, P 〉0.05）. Conclusions VEGFR-1 may play an important role in invasion and metastasis of HCC. Epithelial-mesenchymal transition that induced by VEGFR-1 may contribute to the invasion and metastasis of HCC.