1992～1995年广东省登革热病原学和血清学监测报告

Virological and Serological Surveillance of Dengue Fever in Guangdong 1992～1995

１９９２～１９９５年，在广州市珠海区和海康市各选择１个既往登革热（简称ＤＦ）流行区和非流行区作为ＤＦ监测点，并对省内临床疑似ＤＦ病人进行监测。从１０４份疑似ＤＦ患者急性期血清分离出３４株病毒。分离阳性率为３２．６９％，分别是１９９３年的登革２型和１９９５年的登革１型。１３１６只蚊分为１１６组，经Ｃ６／３６白纹伊蚊细胞培养和单克隆抗体间接免疫荧光试验检测，全部未分离出ＤＦ病毒。１９８份疑似ＤＦ患者血清作登革ＩｇＧ抗体（ＤＶ－ＩｇＧ）检测，检出阳性５５份，检出率为２７．７８％，２份是１９９３年同一患者双相血清。５３份是１９９５年单份血清，与各型ＤＦ病毒抗原呈高度广泛交叉反应，不能判定流行型。５例病后约１００天的ＤＦ患者其ＩｇＧ抗体滴度普遍下降。检测９７２份健康人血清ＤＶ－ＩｇＧ，检出１３１份阳性，检出率为１３．４％，阳性抗体滴度大多在１∶４０。但１９９４年抗体滴度１∶８０～１∶１６０的有１９份，明显高于其它年。

From 1992 to 1995,a previous epidemic area and a non-epidemic area both in Guangzhou Haizhu district and Haikang city were selected as the DF surveillance points.Allsuspected DF patients in wholl province were carried out for tested. 1316 mosquitoes divided into 116 groups were tested and no dengue virus was isolated.The sera of 972 healthy persons were tested for dengue antibody (DV-IgG),and 131 sera of them were positive and the titers of most positive sera were 1∶40.DV-IgG was negative in 3 monkey sera. In the same period,1044 sera of suspected dengue fever patients were tested by mosquito cell culture.34 strains of dengue virus were isolated and identified as serotype 2 in 1993 and serotype 1 in 1995.198 sera samples of suspected dengue cases were tested for DV-IgG and 55 specimens were positive,2 of them were the paired sera from the same patient in 1993. The types of them could not identified due to the severe cross-reaction.