血管紧张素原基因多态性与原发性高血压之间的关系

Association of T704C single nucleotide polymorphism of angiotensinogen gene with essential hypertension

目的探讨血管紧张素原(AGT)基因T704C单核苷酸多态性与原发性高血压之间的关系,寻找可能与高血压有关的遗传标记。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的方法检测中国北方汉族人群AGT基因T704C单核苷酸多态性。结果在研究人群中,AGT基因T、C等位基因频率分别为0.63和0.37,其中患原发性高血压病组T、C等位基因频率分别为0.59和0.41。CC基因型组的原发性高血压患病率达50.00%,明显高于TC基因型组(17.02%)和TT基因型组(16.76%)(P<0.05),此趋势在男性人群中表现更明显(P<0.01)。突变纯合型(CC)与杂合子(TC)、野生型(TT)相比,SBP和DBP值显著升高(P<0.01)。结论携带AGT基因突变纯合子CC基因型个体可能有较高的原发性高血压患病倾向,且在男性人群此趋势更加明显。

[Objective] To investigate the association of the T704C single nueleotide polymorphism of Angiotensinogen gene with essential hypertension in Chinese han populations. And find out new genetic marks about hypertension. [Method] The PCR-RFLR was used to test the T704C polymorphism of sGT （Angiotensinogen gene）. [Results] In the total populations, the frequeney of allele T and C aeeounted for 0.63 and 0.37, respeetively. In the siek group of EH, the frequeney of allele T and C aeeounted for 0.59 and 0.41. The morbidity of individuals earrying CC was greater than that in other groups earrying TC or TY（50.00% vs 17.02%, 50.00% vs 16.76%, P 〈0.05）, espeeially in the groups of ＂male＂ （P 〈0.01）. The level of SBP or DBP in the groups earrying CC genotype was greater than that in other groups carrying TC or Tr genotypes. [Conclusions] There may be some correlation of the mutation type CC with essential hypertension.The morbidity of individuals with mutation type CC is greater than the others,especially in the groups of ＂male＂.