重组大肠杆菌BL21(pBAI)生产人干扰素α2b

Production of Recombinant Human Interferon α2b in Batch and Fed-Batch Culture of Escherichia coli BL21(pBAI)

通过培养重组大肠杆菌BL21(pBAI)表达人干扰素α2b(Human Interferonα2b,hIFNα2b)。hIFNα2b表达由PL启动子控制,通过升温至42°C诱导表达。本研究比较了分批培养和多种补料分批培养方式下hIFNα2b的生产,其中通过恒速流加葡萄糖,hIFNα2b的表达量达到6 540 mg/L,平均生产速率和比速率分别为546 mg/(L.h)和27 mg/(g.h)。升温前1.5 h补充25 g酵母提取物,并以0.27 g/(g.h)的比速率供应葡萄糖,hIFNα2b的平均生产速率达到1 006 mg/(L.h),比生产速率为54 mg/(g.h),对有机氮源的得率提高到138 mg/g。

E. coli BL21 （pBAI） was used for the production of hIFNα2b whose expression was under the control of PL. promoter. Batch and fed batch cultures for hIFNα2b production in complex media were compared at the induction temperature of 42 ℃. By feeding glucose at a constant rate, hIFNα2b production reached 6 540 mg/L, with an overall productivity of 546 mg/（L·h） and an overall specific productivity of 27 mg/（g·h）. By supplying yeast extract before induction and feeding glucose at a specific provision rate of 0.27 g/（g·h）, the overall productivity was as high as 1 006 mg/（L·h）, and the specific productivity reached 54 mg/（g·h） with an overall yield of 138 mg/g based on total complex nitrogen sources.