摘要
目的:探讨地塞米松对宫颈癌Hela细胞裸鼠移植瘤生长及凋亡的作用。方法:BALB/C裸鼠皮下接种宫颈癌Hela细胞。腹腔内注射不同剂量地塞米松15~250mg/kg,测量肿瘤体积观察肿瘤生长情况,用透射电镜及原位细胞凋亡检测(TUNEL)的方法对肿瘤细胞的凋亡进行检测。结果:不同剂量地塞米松体内注射均可抑制宫颈癌Hela细胞裸鼠移植瘤的生长,并能诱导肿瘤细胞凋亡,与对照组相比肿瘤生长速度减慢,体积明显缩小(P〈0.001),凋亡小体明显增多;不同剂量地塞米松对肿瘤生长的影响有差异,当剂量〈62.5mg/kg时,疗效随剂量增加而增加,凋亡小体逐渐增多,肿瘤生长更加缓慢,各组间有明显差异(P〈0.001);剂量为62.5~125me/kg时,肿瘤生长及凋亡发生与剂量无明显的关系;当剂量〉125mg/kg时,疗效随剂量增加而降低,凋亡小体逐渐减少,肿瘤生长速度加快,各组间有明显差异(P〈0.001)。结论:地塞米松体内能诱导裸鼠宫颈癌移植瘤凋亡发生,显著抑制肿瘤生长,并存在一定程度的剂量依赖效应。
Objective: To investigate the effects of dexamethasone on the growth and apoptosis of Hela ceils in cervical transplantation tumor in athymic mouse. Methods: Hela cells were inoculated subcutaneously into BALB/C mice. Different doses of dexamethasone ( 15 - 250 mg / kg) were injected into peritoneum. Volumes of tumor were measured and growth of tumor was observed. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL Results: The tumor sizes in different groups treated by different doses of dexamethasone were significantly smaller than those in the control groups ( P 〈 0. 001 ) . The number of apoptotic bodies in the treated groups were significantly increased than those in the control groups (P 〈 0. 001 ) . In vivo, dexamethasone significantly inhibited tumor growth and induced tumor apoptosis compared with control group ( P 〈 0. 001 ), but different doses had different influences. When doses of dexamethasone were less than 62. 5 mg/kg, the effects increased with the doses accordingly. The apoptotic bodies increased gradually, growth of tumors were slower, and the apoptotic bodies in each group were obviously different (P 〈0. 001 ) . Doses between 62. 5 mg/kg 125 mg/kg there was no significant relationship between tumor growth , apoptosis and doses. When doses were more than 125 mg/kg, with the doses increased, the effects of treatment depressed, the apoptotic bodies reduced and growth of tumors were quicker. It was obviously different in each group ( P 〈 0. 001 ) . Conclusion: Dexamethasone can repress cervical transplantation tumor growth and induce tumor cell apoptosis in athymic mouse in vivo, which has some degree of dose - depended effect.
出处
《中国妇幼保健》
CAS
北大核心
2008年第11期1547-1550,共4页
Maternal and Child Health Care of China