地塞米松诱导裸鼠宫颈癌移植瘤凋亡的实验研究

Apoptosis study of cervical transplantation tumor in athymic mouse induced by dexamethasone

目的：探讨地塞米松对宫颈癌Hela细胞裸鼠移植瘤生长及凋亡的作用。方法：BALB／C裸鼠皮下接种宫颈癌Hela细胞。腹腔内注射不同剂量地塞米松15～250mg／kg，测量肿瘤体积观察肿瘤生长情况，用透射电镜及原位细胞凋亡检测（TUNEL）的方法对肿瘤细胞的凋亡进行检测。结果：不同剂量地塞米松体内注射均可抑制宫颈癌Hela细胞裸鼠移植瘤的生长，并能诱导肿瘤细胞凋亡，与对照组相比肿瘤生长速度减慢，体积明显缩小（P〈0．001），凋亡小体明显增多；不同剂量地塞米松对肿瘤生长的影响有差异，当剂量〈62．5mg／kg时，疗效随剂量增加而增加，凋亡小体逐渐增多，肿瘤生长更加缓慢，各组间有明显差异（P〈0．001）；剂量为62．5～125me／kg时，肿瘤生长及凋亡发生与剂量无明显的关系；当剂量〉125mg／kg时，疗效随剂量增加而降低，凋亡小体逐渐减少，肿瘤生长速度加快，各组间有明显差异（P〈0．001）。结论：地塞米松体内能诱导裸鼠宫颈癌移植瘤凋亡发生，显著抑制肿瘤生长，并存在一定程度的剂量依赖效应。

Objective： To investigate the effects of dexamethasone on the growth and apoptosis of Hela ceils in cervical transplantation tumor in athymic mouse. Methods： Hela cells were inoculated subcutaneously into BALB/C mice. Different doses of dexamethasone （ 15 - 250 mg / kg） were injected into peritoneum. Volumes of tumor were measured and growth of tumor was observed. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL Results： The tumor sizes in different groups treated by different doses of dexamethasone were significantly smaller than those in the control groups （ P 〈 0. 001 ） . The number of apoptotic bodies in the treated groups were significantly increased than those in the control groups （P 〈 0. 001 ） . In vivo, dexamethasone significantly inhibited tumor growth and induced tumor apoptosis compared with control group （ P 〈 0. 001 ）, but different doses had different influences. When doses of dexamethasone were less than 62. 5 mg/kg, the effects increased with the doses accordingly. The apoptotic bodies increased gradually, growth of tumors were slower, and the apoptotic bodies in each group were obviously different （P 〈0. 001 ） . Doses between 62. 5 mg/kg 125 mg/kg there was no significant relationship between tumor growth , apoptosis and doses. When doses were more than 125 mg/kg, with the doses increased, the effects of treatment depressed, the apoptotic bodies reduced and growth of tumors were quicker. It was obviously different in each group （ P 〈 0. 001 ） . Conclusion： Dexamethasone can repress cervical transplantation tumor growth and induce tumor cell apoptosis in athymic mouse in vivo, which has some degree of dose - depended effect.